Madduma Hewage Susara, Prashar Suvira, O Karmin, Siow Yaw L
St. Boniface Hospital Research Centre, Winnipeg, MB R2H 2A6, Canada.
Department of Physiology & Pathophysiology, University of Manitoba, Winnipeg, MB R3E 0J9, Canada.
Antioxidants (Basel). 2021 Apr 6;10(4):565. doi: 10.3390/antiox10040565.
Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease globally and there is a pressing need for effective treatment. Lipotoxicity and oxidative stress are the important mediators in NAFLD pathogenesis. Lingonberry ( L.) is rich in anthocyanins that have antioxidant and anti-inflammatory properties. The present study investigated the effect of lingonberry supplementation on liver injury in C57BL/6J male mice fed a high-fat diet (HFD) for 12 weeks. Mice fed HFD displayed liver injury with steatosis, increased lipid peroxidation and inflammatory cytokine expression in the liver as compared to mice fed a control diet. Lingonberry supplementation for 12 weeks alleviated HFD-induced liver injury, attenuated hepatic lipid accumulation, and inflammatory cytokine expression. Lingonberry supplementation inhibited the expression of sterol regulatory element-binding protein-1c (SREBP-1c) and acetyl-CoA carboxylase-1 (AAC-1) as well as activated AMP-activated protein kinase (AMPK) in the liver. It also decreased HFD-induced hepatic oxidative stress and aggregation of inflammatory foci. This was associated with a restoration of nuclear factor erythroid 2-related factor 2 (Nrf2) and glutathione level in the liver. These results suggest that lingonberry supplementation can protect against HFD-induced liver injury partly through attenuation of hepatic lipid accumulation, oxidative stress, and inflammatory response.
非酒精性脂肪性肝病（NAFLD）是全球最常见的慢性肝病，迫切需要有效的治疗方法。脂毒性和氧化应激是NAFLD发病机制中的重要介质。越橘富含具有抗氧化和抗炎特性的花青素。本研究调查了在12周内给高脂饮食（HFD）喂养的C57BL/6J雄性小鼠补充越橘对肝损伤的影响。与喂食对照饮食的小鼠相比，喂食HFD的小鼠出现肝损伤并伴有脂肪变性，肝脏中的脂质过氧化和炎性细胞因子表达增加。补充越橘12周可减轻HFD诱导的肝损伤，减轻肝脏脂质积累和炎性细胞因子表达。补充越橘可抑制肝脏中固醇调节元件结合蛋白-1c（SREBP-1c）和乙酰辅酶A羧化酶-1（AAC-1）的表达，并激活肝脏中的AMP活化蛋白激酶（AMPK）。它还降低了HFD诱导的肝脏氧化应激和炎性病灶的聚集。这与肝脏中核因子红细胞2相关因子2（Nrf2）和谷胱甘肽水平的恢复有关。这些结果表明，补充越橘可以部分通过减轻肝脏脂质积累、氧化应激和炎症反应来预防HFD诱导的肝损伤。
