Ozaltin Kadir, Di Martino Antonio, Capakova Zdenka, Lehocky Marian, Humpolicek Petr, Saha Tomas, Vesela Daniela, Mozetic Miran, Saha Petr
Center of Polymer Systems, Tomas Bata University in Zlin, Trida Tomase Bati 5678, 760 01 Zlin, Czech Republic.
Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, Lenin Av. 30, 634050 Tomsk, Russia.
Polymers (Basel). 2021 Apr 8;13(8):1201. doi: 10.3390/polym13081201.
The development of antibacterial materials has great importance in avoiding bacterial contamination and the risk of infection for implantable biomaterials. An antibacterial thin film coating on the surface via chemical bonding is a promising technique to keep native bulk material properties unchanged. However, most of the polymeric materials are chemically inert and highly hydrophobic, which makes chemical agent coating challenging Herein, immobilization of chlorhexidine, a broad-spectrum bactericidal cationic compound, onto the polylactic acid surface was performed in a multistep physicochemical method. Direct current plasma was used for surface functionalization, followed by carbodiimide chemistry to link the coupling reagents of N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDAC) and N-Hydroxysuccinimide (NHs) to create a free bonding site to anchor the chlorhexidine. Surface characterizations were performed by water contact angle test, X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). The antibacterial activity was tested using and . Finally, in vitro cytocompatibility of the samples was studied using primary mouse embryonic fibroblast cells. It was found that all samples were cytocompatible and the best antibacterial performance observed was the Chlorhexidine immobilized sample after NHs activation.
抗菌材料的发展对于避免可植入生物材料的细菌污染和感染风险具有重要意义。通过化学键合在表面形成抗菌薄膜涂层是一种有前景的技术，可保持天然块状材料的性能不变。然而，大多数聚合物材料具有化学惰性且高度疏水，这使得化学试剂涂层具有挑战性。在此，采用多步物理化学方法将广谱杀菌阳离子化合物氯己定固定在聚乳酸表面。使用直流等离子体进行表面功能化，然后通过碳二亚胺化学将N-(3-二甲基氨基丙基)-N'-乙基碳二亚胺盐酸盐（EDAC）和N-羟基琥珀酰亚胺（NHs）的偶联试剂连接起来，以创建一个自由键合位点来锚定氯己定。通过水接触角测试、X射线光电子能谱（XPS）和扫描电子显微镜（SEM）进行表面表征。使用[具体方法1]和[具体方法2]测试抗菌活性。最后，使用原代小鼠胚胎成纤维细胞研究样品的体外细胞相容性。结果发现，所有样品都具有细胞相容性，观察到的最佳抗菌性能是NHs活化后的氯己定固定样品。
