College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
University Grenoble Alpes, CEA, CNRS, IRIG, SyMMES, F-38000 Grenoble, France.
Biosensors (Basel). 2021 Apr 8;11(4):112. doi: 10.3390/bios11040112.
Small molecules are ubiquitous in nature and their detection is relevant in various domains. However, due to their size, sensitive and selective probes are difficult to select and the detection methods are generally indirect. In this study, we introduced the use of melting curve analysis of aptachains based on split-aptamers for the detection of adenosine. Aptamers, short oligonucleotides, are known to be particularly efficient probes compared to antibodies thanks to their advantageous probe/target size ratio. Aptachains are formed from dimers with dangling ends followed by the split-aptamer binding triggered by the presence of the target. The high melting temperature of the dimers served as a calibration for the detection/quantification of the target based on the height and/or temperature shift of the aptachain melting peak.
小分子在自然界中无处不在，它们的检测在各个领域都具有重要意义。然而，由于其体积较小，很难选择敏感和选择性的探针，而且检测方法通常是间接的。在本研究中，我们引入了基于分裂适体的适体链的熔解曲线分析，用于检测腺苷。适体是短的寡核苷酸，与抗体相比，由于其有利的探针/靶标大小比，被认为是特别有效的探针。适体链由带有悬垂末端的二聚体形成，然后在目标存在的情况下触发分裂适体结合。二聚体的高熔点温度可作为基于适体链熔解峰的高度和/或温度偏移来检测/定量目标的校准。
