Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, South China University of Technology, Guangzhou, 510640, Guangdong, China.
Anal Bioanal Chem. 2020 Oct;412(25):6655-6665. doi: 10.1007/s00216-020-02754-x. Epub 2020 Jun 29.
The sensitive and selective detection of biomarkers plays a crucial role in disease diagnostics, drug discovery, and early screening of cancers. The achievement of this goal highly depends on the continuous development of biosensing technologies. Among them, fluorescence anisotropy/polarization (FA/FP) analysis receives increasing interest due to the advantage of simple operation, fast response, and no background interference. In recent decades, great progress has been achieved in FA/FP sensors thanks to the development of functional nucleic acids (FNAs) including aptamers and nucleic acid enzymes. This review focuses on FNA-based FA/FP sensors for the quantitative detection of biomarkers, such as nucleic acid, small molecules, and proteins. The design strategies, recognition elements, and practical applications are fully highlighted. The article also discusses the challenges of applying FNA-based FA/FP sensors in the next generation and the potential solutions along with future prospects. Graphical abstract.
生物标志物的灵敏和选择性检测在疾病诊断、药物发现和癌症早期筛查中起着至关重要的作用。这一目标的实现高度依赖于生物传感技术的不断发展。在这些技术中，由于操作简单、响应快速且无背景干扰等优点，荧光各向异性/偏振（FA/FP）分析受到越来越多的关注。在过去的几十年中，得益于功能核酸（FNAs），包括适体和核酸酶的发展，FA/FP 传感器取得了巨大的进展。本文重点介绍了基于 FNAs 的 FA/FP 传感器在定量检测生物标志物（如核酸、小分子和蛋白质）方面的应用。充分强调了设计策略、识别元件和实际应用。本文还讨论了将基于 FNA 的 FA/FP 传感器应用于下一代技术所面临的挑战以及潜在的解决方案和未来展望。
