Pham Xuan-Hung, Seong Bomi, Hahm Eunil, Huynh Kim-Hung, Kim Yoon-Hee, Kim Jaehi, Lee Sang Hun, Jun Bong-Hyun
Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea.
Department of Chemical and Biological Engineering, Hanbat National University, Daejeon 34158, Korea.
Nanomaterials (Basel). 2021 Apr 8;11(4):948. doi: 10.3390/nano11040948.
The importance of glucose in many biological processes continues to garner increasing research interest in the design and development of efficient biotechnology for the sensitive and selective monitoring of glucose. Here we report on a surface-enhanced Raman scattering (SERS) detection of 4-mercaptophenyl boronic acid (4-MPBA)-immobilized gold-silver core-shell assembled silica nanostructure (SiO@Au@Ag@4-MPBA) for quantitative, selective detection of glucose in physiologically relevant concentration. This work confirmed that 4-MPBA converted to 4-mercaptophenol (4-MPhOH) in the presence of HO. In addition, a calibration curve for HO detection of 0.3 µg/mL was successfully detected in the range of 1.0 to 1000 µg/mL. Moreover, the SiO@Au@Ag@4-MPBA for glucose detection was developed in the presence of glucose oxidase (GOx) at the optimized condition of 100 µg/mL GOx with 1-h incubation time using 20 µg/mL SiO@Au@Ag@4-MPBA and measuring Raman signal at 67 µg/mL SiO@Au@Ag. At the optimized condition, the calibration curve in the range of 0.5 to 8.0 mM was successfully developed with an LOD of 0.15 mM. Based on those strategies, the SERS detection of glucose can be achieved in the physiologically relevant concentration range and opened a great promise to develop a SERS-based biosensor for a variety of biomedicine applications.
葡萄糖在许多生物过程中的重要性，持续引发人们对设计和开发高效生物技术以灵敏且选择性地监测葡萄糖的研究兴趣。在此，我们报道了一种表面增强拉曼散射（SERS）检测方法，用于检测固定有4-巯基苯硼酸（4-MPBA）的金-银核壳组装二氧化硅纳米结构（SiO@Au@Ag@4-MPBA），以定量、选择性地检测生理相关浓度的葡萄糖。这项工作证实了4-MPBA在HO存在下会转化为4-巯基苯酚（4-MPhOH）。此外，在1.0至1000μg/mL范围内成功检测到了HO检测的校准曲线，其浓度为0.3μg/mL。而且，在葡萄糖氧化酶（GOx）存在的情况下，在100μg/mL GOx、1小时孵育时间的优化条件下，使用20μg/mL SiO@Au@Ag@4-MPBA并在67μg/mL SiO@Au@Ag处测量拉曼信号，开发了用于葡萄糖检测的SiO@Au@Ag@4-MPBA。在优化条件下，成功绘制了0.5至8.0 mM范围内的校准曲线，检测限为0.15 mM。基于这些策略，可以在生理相关浓度范围内实现葡萄糖的SERS检测，并为开发用于各种生物医学应用的基于SERS的生物传感器带来了巨大希望。
