Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-bioengineering/College of Life Sciences, Guizhou University, Guiyang 550025, China; Guizhou Key Laboratory of Agro-Bioengineering, Institute of Agro-bioengineering/College of Life Sciences, Guiyang 550025, Guizhou Province, China; National-Local Joint Engineering Research Center of Karst Region Plant Resources Utilization & Breeding (Guizhou), Guiyang 550025, Guizhou Province, China.
Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-bioengineering/College of Life Sciences, Guizhou University, Guiyang 550025, China; Institute for Forest Resources & Environment of Guizhou/ College of Forestry, Guizhou University, Guiyang 550025, China.
Gene. 2024 Apr 30;904:148164. doi: 10.1016/j.gene.2024.148164. Epub 2024 Jan 13.
C2H2-type zinc finger proteins are one of the most widely studied families in plants and play important roles in abiotic stress responses. In the present study, the physicochemical properties, chromosomal locations, evolutionary relationships, and gene structures of 54 C2H2 zinc finger protein (ZFP) family members were analyzed in apple. The MdC2H2-ZFP genes were phylogenetically clustered into seven subfamilies distributed in different densities on 16 chromosomes. The RNA-seq data from various tissues revealed that MdC2H2-ZFPs differentially expressed among root, stem, leaf, flower, and fruits. Quantitative analysis of its expression characteristics showed that the MdC2H2-ZFP genes were rapidly induced as exposure to abiotic stresses such as drought, salt and low temperature etc. Under drought stress, the expression of eight members was significantly up-regulated, and the highest was obtained from MdC2H2-17; as exposure to salt stress, nine MdC2H2-ZFPs was obviously up-regulated, with the highest expression of MdC2H2-13; and under low temperature stress, the expression of seven members was highly up-regulated, and MdC2H2-13 also demonstrated the highest expression which is same as the case under salt stress. Therefore, some members of MdC2H2-ZFP gene family considerably involve in the multiple abiotic stress responses, which may better understand the function of this family and facilitate the breeding of apple for stress tolerance.
C2H2 型锌指蛋白是植物中研究最广泛的家族之一，在非生物胁迫反应中发挥重要作用。本研究分析了苹果中 54 个 C2H2 锌指蛋白（ZFP）家族成员的理化性质、染色体位置、进化关系和基因结构。MdC2H2-ZFP 基因在 16 条染色体上以不同密度聚类为七个亚家族。来自不同组织的 RNA-seq 数据显示，MdC2H2-ZFPs 在根、茎、叶、花和果实中差异表达。对其表达特性的定量分析表明，MdC2H2-ZFP 基因在受到干旱、盐和低温等非生物胁迫时迅速诱导表达。在干旱胁迫下，8 个成员的表达明显上调，其中 MdC2H2-17 的表达最高；在盐胁迫下，9 个 MdC2H2-ZFPs 明显上调，MdC2H2-13 的表达最高；在低温胁迫下，7 个成员的表达高度上调，MdC2H2-13 的表达也与盐胁迫时相同最高。因此，MdC2H2-ZFP 基因家族的一些成员参与了多种非生物胁迫反应，这可能有助于更好地理解该家族的功能，并促进苹果的抗逆性育种。
