Laboratorio de Química Farmacéutica, Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, Ciudad Universitaria, San Nicolás de los Garza, Nuevo León 66451, Mexico.
Centro de Investigación en Nutrición y Salud Publica, Facultad de Salud Pública y Nutrición, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León 66460, Mexico.
Molecules. 2021 Apr 11;26(8):2200. doi: 10.3390/molecules26082200.
The bioassay-guided fractionation of a CHCl-MeOH extract from the stems of identified an active fraction against PC3 prostate cancer cells. The treatment for 24 h showed an 80% reduction in cell viability ( ≤ 0.05) by a WST-1 assay at a concentration of 100 μg/mL. The HPLC-QTOF-MS analysis of the fraction showed the presence of coumaric and isoferulic acids, apigenin, kaempferol, chrysoeriol, naringenin, ursolic and betulinic acids, hexadecadienoic and octadecadienoic fatty acids, and the stilbene resveratrol. The exposure of PC3 cells to resveratrol (IC = 23 μg/mL) for 24 h induced significant changes in 847 genes (Z-score ≥ ±2). The functional classification tool of the DAVID v6.8 platform indicates that the underlying molecular mechanisms against the proliferation of PC3 cells were associated ( ≤ 0.05) with the process of differentiation and metabolism. These findings provide experimental evidence suggesting the potential of as a promising natural source of anticancer compounds.
从 茎部的 CHCl-MeOH 提取物中进行生物测定指导的分段，鉴定出一种对 PC3 前列腺癌细胞具有活性的部分。WST-1 测定法在浓度为 100 μg/mL 时，24 h 的治疗显示细胞活力降低 80%（≤0.05）。该部分的 HPLC-QTOF-MS 分析表明存在咖啡酸和异阿魏酸、芹菜素、山奈酚、白杨素、柚皮素、熊果酸和齐墩果酸、十六碳二烯酸和十八碳二烯酸脂肪酸，以及白藜芦醇。将 PC3 细胞暴露于白藜芦醇（IC=23μg/mL）24 h 会引起 847 个基因的显著变化（Z 评分≥±2）。DAVID v6.8 平台的功能分类工具表明，针对 PC3 细胞增殖的潜在分子机制与分化和代谢过程相关（≤0.05）。这些发现为 作为有前途的抗癌化合物天然来源提供了实验证据。
