Haubek Dorte, Mulli Tonnie, Kemoli Arthur, Lindholm Mark, Gjørup Hans, Nørregaard Marie-Louise Milvang, Johansson Anders
Section for Paediatric Dentistry, Department of Dentistry and Oral Health, Health, Aarhus University, DK-8000 Aarhus, Denmark.
Department of Periodontology, Community and Preventive Dentistry, University of Nairobi, P.O. Box 34848, Nairobi 00100, Kenya.
Pathogens. 2021 Apr 17;10(4):488. doi: 10.3390/pathogens10040488.
is implicated in the etiology of periodontitis that affects adolescents. The monitoring and mapping of the geographic dissemination pattern of JP2 and non-JP2 genotypes of are of interest. In Africa, the highly leukotoxic JP2 genotype is known to be prevalent, particularly in north-west Africa. The aims of this study were to determine the prevalence of JP2 and non-JP2 genotypes and investigate the oral hygiene practices among adolescents living in Maasai Mara, Kenya. A total of 284 adolescents (mean age: 15.0 yrs; SD 1.1) were interviewed regarding their age, gender, medical history, and oral hygiene practice, and the number of teeth present was recorded. One subgingival pooled plaque sample from all the first molars of each participant was analyzed by conventional PCR. The mean number of permanent teeth present was 27.9 (SD: 2.0; range: 22-32; 95% CI: 27.7-28.1). Sixteen (5.6%) and two (0.7%) adolescents were positive for non-JP2 and JP2 genotypes, respectively. For the vast majority of the adolescents, the use of a toothbrush (99.3%) and toothpaste (80.1%), as well as some kind of toothpick (>60.2%), were part of their oral hygiene practice, with dental floss (0.4%) and/or mouth rinses (0.4%) rarely being used. We have, for the first time, identified Kenyan adolescents colonized with the JP2 genotype. The prevalence of the JP2 genotype of is low, a possible indicator that it spreading through human migration from North and West Africa to East Africa is a rare occasion.
与影响青少年的牙周炎病因有关。监测和绘制的JP2和非JP2基因型的地理传播模式很有意义。在非洲，已知具有高度白细胞毒性的JP2基因型很普遍，特别是在非洲西北部。本研究的目的是确定肯尼亚马赛马拉地区青少年中JP2和非JP2基因型的流行情况，并调查他们的口腔卫生习惯。共对284名青少年（平均年龄：15.0岁；标准差1.1）进行了访谈，询问他们的年龄、性别、病史和口腔卫生习惯，并记录现存牙齿数量。通过常规PCR分析了每个参与者所有第一磨牙的一个龈下菌斑混合样本。现存恒牙的平均数量为27.9颗（标准差：2.0；范围：22 - 32；95%置信区间：27.7 - 28.1）。分别有16名（5.6%）和2名（0.7%）青少年的非JP2和JP2基因型呈阳性。对于绝大多数青少年来说，使用牙刷（99.3%）和牙膏（80.1%）以及某种牙签（>60.2%）是他们口腔卫生习惯的一部分，很少使用牙线（0.4%）和/或漱口水（0.4%）。我们首次发现了携带JP2基因型的肯尼亚青少年。的JP2基因型流行率较低，这可能表明它通过人类从北非和西非向东非迁移而传播的情况很少见。
