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静相偏光结构光照明显微镜

Motionless Polarizing Structured Illumination Microscopy.

机构信息

3D Optical Metrology Laboratory, Department of Photonic Engineering, Chosun University, 309 Pilmun-Daero, Dong-gu, Gwangju 61452, Korea.

出版信息

Sensors (Basel). 2021 Apr 17;21(8):2837. doi: 10.3390/s21082837.

DOI:10.3390/s21082837
PMID:33920615
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8073734/
Abstract

In this investigation, we propose a motionless polarizing structured illumination microscopy as an axially sectioning and reflective-type device to measure the 3D surface profiles of specimens. Based on the spatial phase-shifting technique to obtain the visibility of the illumination pattern. Instead of using a grid, a Wollaston prism is used to generate the light pattern by the stable interference of two beams. As the polarization states of two beams are orthogonal with each other, a polarization pixelated CMOS camera can simultaneously obtain four phase-shifted patterns with the beams after passing through a quarter wave plate based on the spatial phase-shifting technique with polarization. In addition, a focus tunable lens is used to eliminate a mechanical moving part for the axial scanning of the specimen. In the experimental result, a step height sample and a concave mirror were measured with 0.05 µm and 0.2 mm repeatabilities of step height and the radius of curvature, respectively.

摘要

在这项研究中,我们提出了一种静止的偏光结构照明显微镜,作为一种轴向切片和反射式的装置,用于测量样品的三维表面轮廓。基于空间相移技术获得照明图案的可见度。我们使用渥拉斯顿棱镜代替网格来生成光图案,渥拉斯顿棱镜通过两束光的稳定干涉产生光图案。由于两束光的偏振态彼此正交,因此偏振分像素 CMOS 相机可以通过空间相移技术与偏振一起,在通过四分之一波片后同时获得四个相移图案。此外,我们使用调焦可调透镜消除了用于样品轴向扫描的机械移动部件。在实验结果中,我们对一个台阶高度样品和一个凹面镜进行了测量,台阶高度的重复性为 0.05 µm,曲率半径的重复性为 0.2 mm。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/12b82f068a91/sensors-21-02837-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/2f0ba8041ee3/sensors-21-02837-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/019a56cec139/sensors-21-02837-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/f06954564b51/sensors-21-02837-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/32a4033befc1/sensors-21-02837-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/4776372ea40d/sensors-21-02837-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/c30219055887/sensors-21-02837-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/62300fb1fd0d/sensors-21-02837-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/e8f9e234b2dd/sensors-21-02837-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/75374c897b2f/sensors-21-02837-g009a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/6b2ff2091ecd/sensors-21-02837-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/8d2fbcfc59e8/sensors-21-02837-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/814f712063ad/sensors-21-02837-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/12b82f068a91/sensors-21-02837-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/2f0ba8041ee3/sensors-21-02837-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/019a56cec139/sensors-21-02837-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/f06954564b51/sensors-21-02837-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/32a4033befc1/sensors-21-02837-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/4776372ea40d/sensors-21-02837-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/c30219055887/sensors-21-02837-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/62300fb1fd0d/sensors-21-02837-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/e8f9e234b2dd/sensors-21-02837-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/75374c897b2f/sensors-21-02837-g009a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/6b2ff2091ecd/sensors-21-02837-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/8d2fbcfc59e8/sensors-21-02837-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/814f712063ad/sensors-21-02837-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb68/8073734/12b82f068a91/sensors-21-02837-g013.jpg

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本文引用的文献

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Improved computer-generated moiré profilometry with flat image calibration.通过平面图像校准改进计算机生成的莫尔轮廓术。
Appl Opt. 2021 Feb 10;60(5):1209-1216. doi: 10.1364/AO.412291.
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Fast TIRF-SIM imaging of dynamic, low-fluorescent biological samples.动态、低荧光生物样本的快速全内反射结构光照明显微镜成像
Biomed Opt Express. 2020 Jun 26;11(7):4008-4026. doi: 10.1364/BOE.391561. eCollection 2020 Jul 1.
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