Medical Biochemistry Department, Faculty of Human Medicine, Zagazig University, Zagazig 44523, Egypt.
General Surgery Department, Faculty of Human Medicine, Zagazig University, Zagazig 44523, Egypt.
Genes (Basel). 2021 Apr 19;12(4):598. doi: 10.3390/genes12040598.
Early detection of colorectal cancer (CRC) is the most important factor in deciding its prognosis, so the need to develop an accurate screening test is a must. P-element induced wimpy testis (PIWI) RNA-823 (piR-823) is one of the first piRNAs recognized to be linked to malignancy. We aimed to investigate the expression levels of piR-823 in both serum and tissues of colorectal cancer patients and the ability to use its serum level as a non-invasive diagnostic biomarker to detect colorectal cancer. We determined piR-823 expression levels in 84 serum samples of CRC patients, 75 serum samples of healthy controls, and biological specimens obtained from the 84 patients with colorectal cancer from both the tumor tissues and the normal neighboring tissues using quantitative real-time reverse transcriptase-PCR. We showed that piR-823 had significantly higher serum and tissue expression levels in CRC patients compared to the controls. We observed a significant positive correlation between piR-823 serum levels and the staging of CRC, with significantly higher levels exhibiting advanced stages of CRC (III and IV). This translates into poorer differentiation and lymph node metastasis. The receiver operating characteristic curve (ROC curve) test showed 83.3% sensitivity and 89.3% specificity at a cut-off value of >5.98-fold change, with an area under the curve of 0.933 ( < 0.0001) concerning the ability of piR-823 in diagnosing patients with colorectal carcinoma. piR-823 expression is upregulated in colorectal cancer patients' serum and tissues, and it can be used as a diagnostic noninvasive biomarker for CRC.
结直肠癌（CRC）的早期检测是决定其预后的最重要因素，因此必须开发一种准确的筛查试验。P 元件诱导的软弱(testis, PIWI) RNA-823（piR-823）是最早被认为与恶性肿瘤相关的 piRNA 之一。我们旨在研究 piR-823 在结直肠癌患者血清和组织中的表达水平，以及将其血清水平用作非侵入性诊断生物标志物来检测结直肠癌的能力。我们使用定量实时逆转录-PCR 测定了 84 例结直肠癌患者、75 例健康对照者的血清样本以及 84 例结直肠癌患者肿瘤组织和正常邻近组织的生物样本中 piR-823 的表达水平。我们发现，与对照组相比，piR-823 在 CRC 患者的血清和组织中表达水平显著升高。我们观察到 piR-823 血清水平与 CRC 的分期之间存在显著的正相关，晚期（III 和 IV 期）CRC 患者的水平显著升高。这反映在分化较差和淋巴结转移。受试者工作特征曲线（ROC 曲线）试验显示，在截断值>5.98 倍变化时，piR-823 对诊断结直肠癌患者的敏感性为 83.3%，特异性为 89.3%，曲线下面积为 0.933（<0.0001）。piR-823 在结直肠癌患者的血清和组织中表达上调，可作为 CRC 的诊断性非侵入性生物标志物。
