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用于检测六种呼吸道细菌病原体的耐热七重PCR检测法

Thermostable Heptaplex PCR Assay for the Detection of Six Respiratory Bacterial Pathogens.

作者信息

Nik Zuraina Nik Mohd Noor, Goni Mohammed Dauda, Amalina Khazani Nur, Hasan Habsah, Mohamad Suharni, Suraiya Siti

机构信息

Department of Medical Microbiology and Parasitology, Universiti Sains Malaysia, Kota Bharu 16150, Kelantan, Malaysia.

Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu 16100, Kelantan, Malaysia.

出版信息

Diagnostics (Basel). 2021 Apr 22;11(5):753. doi: 10.3390/diagnostics11050753.

DOI:10.3390/diagnostics11050753
PMID:33922299
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8146555/
Abstract

A thermostabilized, multiplex polymerase chain reaction (mPCR) assay was developed in this study for the detection of six respiratory bacterial pathogens. Specific primers were designed for an internal amplification control (IAC) and six target sequences from , , , , , and . The resultant seven-band positive amplification control (PAC) of this heptaplex PCR assay corresponded to 105 base pairs (bp) of IAC, 202 bp of , 293 bp of , 349 bp of , 444 bp of , 505 bp of , and 582 bp of . Results found that 6% (/) of the stabilizer was optimum to preserve the functional conformation of DNA polymerase enzyme. This assay was stable at ambient temperature for at least 6 months. The sensitivity and specificity of this assay were both 100% when testing on the intended target organisms ( = 119) and non-intended species ( = 57). The mPCR assay developed in this study enabled accurate, rapid, and simple detection of six respiratory bacteria.

摘要

本研究开发了一种热稳定的多重聚合酶链反应（mPCR）检测方法，用于检测六种呼吸道细菌病原体。针对内部扩增对照（IAC）以及来自[具体菌名1]、[具体菌名2]、[具体菌名3]、[具体菌名4]、[具体菌名5]和[具体菌名6]的六个靶序列设计了特异性引物。这种七重PCR检测方法产生的七带阳性扩增对照（PAC）分别对应IAC的105个碱基对（bp）、[具体菌名1]的202 bp、[具体菌名2]的293 bp、[具体菌名3]的349 bp、[具体菌名4]的444 bp、[具体菌名5]的505 bp和[具体菌名6]的582 bp。结果发现，6%（/）的稳定剂最适合保持DNA聚合酶的功能构象。该检测方法在环境温度下至少6个月稳定。在对预期靶标生物（n = 119）和非预期物种（n = 57）进行检测时，该检测方法的灵敏度和特异性均为100%。本研究开发的mPCR检测方法能够准确、快速且简单地检测六种呼吸道细菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e678/8146555/60f945bf84ae/diagnostics-11-00753-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e678/8146555/4d4d23c851ba/diagnostics-11-00753-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e678/8146555/f12932fc29fe/diagnostics-11-00753-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e678/8146555/1e1c3648d9cf/diagnostics-11-00753-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e678/8146555/60f945bf84ae/diagnostics-11-00753-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e678/8146555/4d4d23c851ba/diagnostics-11-00753-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e678/8146555/f12932fc29fe/diagnostics-11-00753-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e678/8146555/1e1c3648d9cf/diagnostics-11-00753-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e678/8146555/60f945bf84ae/diagnostics-11-00753-g004.jpg

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2
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Int J Infect Dis. 2016 Dec;53:15-20. doi: 10.1016/j.ijid.2016.10.005. Epub 2016 Oct 21.
3
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4
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9
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