呼吸道感染病原体芯片
Pathogen chip for respiratory tract infections.
机构信息
University of Colorado School of Medicine, Aurora, Colorado, USA.
出版信息
J Clin Microbiol. 2013 Mar;51(3):945-53. doi: 10.1128/JCM.02317-12. Epub 2013 Jan 9.
Determining the viral etiology of respiratory tract infections (RTI) has been limited for the most part to specific primer PCR-based methods due to their increased sensitivity and specificity compared to other methods, such as tissue culture. However, specific primer approaches have limited the ability to fully understand the diversity of infecting pathogens. A pathogen chip system (PathChip), developed at the Genome Institute of Singapore (GIS), using a random-tagged PCR coupled to a chip with over 170,000 probes, has the potential to recognize all known human viral pathogens. We tested 290 nasal wash specimens from Filipino children <2 years of age with respiratory tract infections using culture and 3 PCR methods-EraGen, Luminex, and the GIS PathChip. The PathChip had good diagnostic accuracy, ranging from 85.9% (95% confidence interval [CI], 81.3 to 89.7%) for rhinovirus/enteroviruses to 98.6% (95% CI, 96.5 to 99.6%) for PIV 2, compared to the other methods and additionally identified a number of viruses not detected by these methods.
确定呼吸道感染（RTI）的病毒病因在很大程度上仅限于特定引物 PCR 方法，因为与组织培养等其他方法相比，它们具有更高的灵敏度和特异性。然而，特定引物方法限制了全面了解感染病原体多样性的能力。新加坡基因组研究所（GIS）开发的病原体芯片系统（PathChip）使用随机标记 PCR 与带有超过 170,000 个探针的芯片相结合，具有识别所有已知人类病毒病原体的潜力。我们使用培养和 3 种 PCR 方法（EraGen、Luminex 和 GIS PathChip）测试了 290 名菲律宾 2 岁以下呼吸道感染儿童的鼻洗液标本。PathChip 的诊断准确性良好，范围从鼻病毒/肠道病毒的 85.9%（95%置信区间[CI]，81.3 至 89.7%）到 PIV2 的 98.6%（95%CI，96.5 至 99.6%），与其他方法相比，并且还鉴定了这些方法未检测到的一些病毒。
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