State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, Engineering Research Center for Plant Biotechnology and Germplasm Utilization of Ministry of Education, College of Life Science, Wuhan University, Wuhan 430072, China.
School of Biological Sciences, University of Western Australia, Perth 6009, Australia.
Genes (Basel). 2021 Apr 23;12(5):634. doi: 10.3390/genes12050634.
Sulfate transporters (SULTRs), also known as H/SO symporters, play a key role in sulfate transport, plant growth and stress responses. However, the evolutionary relationships and functional differentiation of SULTRs in Gramineae crops are rarely reported. Here, 111 SULTRs were retrieved from the genomes of 10 Gramineae species, including , , , , , , , and ( ssp. indica and ssp. japonica). The SULTRs were clustered into five clades based on a phylogenetic analysis. Syntheny analysis indicates that whole-genome duplication/segmental duplication and tandem duplication events were essential in the SULTRs family expansion. We further found that different clades and orthologous groups of SULTRs were under a strong purifying selective force. Expression analysis showed that rice SULTRs with high-affinity transporters are associated with the functions of sulfate uptake and transport during rice seedling development. Furthermore, using ssp. indica as a model species, we found that was significantly upregulated under salt stress, while and showed remarkable upregulation under high temperature, low-selenium and drought stresses. and were upregulated under both low-selenium and high-selenium stresses. This study illustrates the expression and evolutionary patterns of the SULTRs family in Gramineae species, which will facilitate further studies of SULTR in other Gramineae species.
硫酸盐转运蛋白（SULTRs），也称为 H/SO 共转运蛋白，在硫酸盐运输、植物生长和应激反应中发挥着关键作用。然而，禾本科作物中 SULTRs 的进化关系和功能分化很少有报道。在这里，从 10 种禾本科物种的基因组中检索到 111 个 SULTRs，包括 、 、 、 、 、 、 、 和 （ssp. indica 和 ssp. japonica）。基于系统发育分析，SULTRs 被聚类为五个分支。共线性分析表明，全基因组复制/片段复制和串联复制事件是 SULTRs 家族扩张的关键。我们进一步发现，不同分支和直系同源群的 SULTRs 受到强烈的纯化选择压力。表达分析表明，与水稻幼苗发育过程中硫酸盐吸收和转运功能相关的水稻 SULTRs 具有高亲和力转运蛋白。此外，以 ssp. indica 为模式物种，我们发现 在盐胁迫下显著上调，而 和 在高温、低硒和干旱胁迫下表现出明显上调。 和 在低硒和高硒胁迫下均上调。本研究说明了禾本科物种中 SULTRs 家族的表达和进化模式，这将有助于进一步研究其他禾本科物种中的 SULTR。
