Redman Regina S, Kim Yong Ok, Cho Sang, Mercer Malia, Rienstra Melissa, Manglona Ryan, Biaggi Taylor, Zhou Xin-Gen, Chilvers Martin, Gray Zachery, Rodriguez Russell J
Adaptive Symbiotic Technologies, Seattle, WA 98105-5663, USA.
Texas A&M AgriLife Research Center, Beaumont, TX 77713, USA.
Microorganisms. 2021 Apr 25;9(5):920. doi: 10.3390/microorganisms9050920.
Studies were undertaken to determine if fungal endophytes from plants in stressful habitats could be commercialized to generate climate resilient crop plants. Fungal endophytes were isolated from weedy rice plants and grasses from South Korea and the USA, respectively. Endophytes ( and ) from weedy rice plants from high salt or drought stressed habitats in South Korea conferred salt and drought stress tolerance to weedy rice and commercial varieties reflective of the habitats from which they were isolated. Fungal endophytes isolated from grasses in arid habitats of the USA were identified as and conferred drought and heat stress tolerance to monocots and eudicots. Two isolates were exposed to UV mutagenesis to derive strains resistant to fungicides in seed treatment plant protection packages. Three strains that collectively had resistance to commonly used fungicides were used for field testing. The three-strain mixture (ThSM3a) increased crop yields proportionally to the level of stress plants experienced with average yields up to 52% under high and 3-5% in low stress conditions. This study demonstrates fungal endophytes can be developed as viable commercial tools for rapidly generating climate resilient crops to enhance agricultural sustainability.
开展了多项研究，以确定来自恶劣生境中植物的真菌内生菌是否能够商业化，从而培育出适应气候变化的作物。分别从韩国和美国的杂草稻植株和禾本科植物中分离出真菌内生菌。从韩国高盐或干旱胁迫生境的杂草稻植株中分离出的内生菌（和）赋予了杂草稻和反映其分离生境的商业品种耐盐和耐旱胁迫能力。从美国干旱生境的禾本科植物中分离出的真菌内生菌被鉴定为，并赋予单子叶植物和双子叶植物耐旱和耐热胁迫能力。将两株分离株进行紫外线诱变，以获得对种子处理植物保护包装中的杀菌剂具有抗性的菌株。使用了三株对常用杀菌剂具有抗性的菌株进行田间试验。三菌株混合物（ThSM3a）使作物产量与植株所经历的胁迫水平成比例增加，在高胁迫条件下平均产量提高了52%，在低胁迫条件下提高了3 - 5%。这项研究表明，真菌内生菌可作为可行的商业工具来快速培育适应气候变化的作物，以增强农业可持续性。
