Baranowska-Wójcik Ewa, Gustaw Klaudia, Szwajgier Dominik, Oleszczuk Patryk, Pawlikowska-Pawlęga Bożena, Pawelec Jarosław, Kapral-Piotrowska Justyna
Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences, Skromna Street 8, 20-704 Lublin, Poland.
Department of Radiochemistry and Environmental Chemistry, Faculty of Chemistry, Maria Curie-Skłodowska University, 20-031 Lublin, Poland.
Foods. 2021 Apr 25;10(5):939. doi: 10.3390/foods10050939.
Food-grade titanium dioxide (TiO) containing a nanoparticle fraction (TiO NPs -nanoparticles) is widely used as a food additive (E171 in the EU). In recent years, it has increasingly been raising controversies as to the presence or absence of its harmful effects on the gastrointestinal microbiota. The complexity and variability of microbiota species present in the human gastrointestinal tract impede the assessment of the impact of food additives on this ecosystem. As unicellular organisms, bacteria are a very convenient research model for investigation of the toxicity of nanoparticles. We examined the effect of TiO (three types of food-grade E171 and one TiO NPs, 21 nm) on the growth of 17 strains of lactic acid bacteria colonizing the human digestive tract. Each bacterial strain was treated with TiO at four concentrations (60, 150, 300, and 600 mg/L TiO). The differences in the growth of the individual strains were caused by the type and concentration of TiO. It was shown that the growth of a majority of the analyzed strains was decreased by the application of E171 and TiO NPs already at the concentration of 150 and 300 mg/L. At the highest dose (600 mg/L) of the nanoparticles, the reactions of the bacteria to the different TiO types used in the experiment varied.
含有纳米颗粒部分（二氧化钛纳米颗粒-TiO NPs）的食品级二氧化钛（TiO₂）被广泛用作食品添加剂（在欧盟为E171）。近年来，关于其对胃肠道微生物群是否存在有害影响的争议日益增多。人类胃肠道中存在的微生物种类的复杂性和变异性阻碍了对食品添加剂对该生态系统影响的评估。作为单细胞生物，细菌是研究纳米颗粒毒性的非常方便的研究模型。我们研究了TiO₂（三种食品级E171和一种21纳米的TiO NPs）对17种定殖于人类消化道的乳酸菌菌株生长的影响。每种细菌菌株用四种浓度（60、150、300和600毫克/升TiO₂）的TiO₂处理。各菌株生长的差异是由TiO₂的类型和浓度引起的。结果表明，在150和300毫克/升的浓度下，应用E171和TiO NPs已经使大多数分析菌株的生长下降。在纳米颗粒的最高剂量（600毫克/升）下，细菌对实验中使用的不同TiO₂类型的反应各不相同。
