Prates José A M, Freire João P B, de Almeida André M, Martins Cátia, Ribeiro David M, Osório Hugo, Pinho Mário A S, Lopes Paula A, Correia Jorge M J, Pinto Rui M A, Costa Teresa, Corrent Etienne, Chalvon-Demersay Tristan
CIISA-Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, Alto da Ajuda, 1300-477 Lisboa, Portugal.
LEAF-Linking Engineering, Agriculture and Food, Departamento de Ciências e Engenharia de Biossistemas, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
Animals (Basel). 2021 Apr 16;11(4):1143. doi: 10.3390/ani11041143.
In order to investigate the effect of a dietary amino acid mixture supplementation in lipopolysaccharide (LPS)-challenged weaned piglets, twenty-seven 28-day-old (8.2 ± 1.0 kg) newly weaned piglets were randomly allocated to one of three experimental treatments for five weeks. Diet 1: a CTRL treatment. Diet 2: an LPS treatment, where piglets were intraperitoneally administered LPS (25 μg/kg) on day 7. Diet 3: an LPS+MIX treatment, where piglets were intraperitoneally administered LPS on day 7 and fed a diet supplemented with a mixture of 0.3% of arginine, branched-chain amino acids (leucine, valine, and isoleucine), and cystine (MIX). Blood samples were drawn on day 10 and day 35, and serum was analysed for selected chemical parameters and proteomics. The LPS and LPS+MIX groups exhibited an increase in haptoglobin concentrations on day 10. The LPS group showed an increased cortisol concentration, while this concentration was reduced in the LPS+MIX group compared to the control group. Similarly, the LPS+MIX group showed a decreased haptoglobin concentration on day 35 compared to the two other groups. Immunoglobulin concentrations were affected by treatments. Indeed, on day 10, the concentrations of IgG and IgM were decreased by the LPS challenge, as illustrated by the lower concentrations of these two immunoglobulins in the LPS group compared to the control group. In addition, the supplementation with the amino acid mixture in the LPS+MIX further decreased IgG and increased IgM concentrations compared to the LPS group. Although a proteomics approach did not reveal important alterations in the protein profile in response to treatments, LPS-challenged piglets had an increase in proteins linked to the immune response, when compared to piglets supplemented with the amino acid mixture. Overall, data indicate that LPS-challenged piglets supplemented with this amino acid mixture are more protected against the detrimental effects of LPS.
为了研究日粮氨基酸混合物补充剂对脂多糖（LPS）刺激的断奶仔猪的影响，将27头28日龄（8.2±1.0千克）的新断奶仔猪随机分为三种实验处理之一，为期五周。日粮1：对照处理。日粮2：LPS处理，仔猪在第7天腹腔注射LPS（25微克/千克）。日粮3：LPS+MIX处理，仔猪在第7天腹腔注射LPS，并饲喂补充有0.3%精氨酸、支链氨基酸（亮氨酸、缬氨酸和异亮氨酸）和胱氨酸混合物（MIX）的日粮。在第10天和第35天采集血样，并分析血清中的选定化学参数和蛋白质组学。LPS组和LPS+MIX组在第10天触珠蛋白浓度升高。LPS组皮质醇浓度升高，而LPS+MIX组与对照组相比该浓度降低。同样，与其他两组相比，LPS+MIX组在第35天触珠蛋白浓度降低。免疫球蛋白浓度受处理影响。实际上，在第10天，LPS刺激使IgG和IgM浓度降低，如LPS组这两种免疫球蛋白的浓度低于对照组所示。此外，与LPS组相比，LPS+MIX组补充氨基酸混合物后IgG进一步降低，IgM浓度升高。尽管蛋白质组学方法未揭示处理后蛋白质谱的重要变化，但与补充氨基酸混合物的仔猪相比，LPS刺激的仔猪与免疫反应相关的蛋白质增加。总体而言，数据表明，补充这种氨基酸混合物的LPS刺激仔猪对LPS的有害影响具有更强的抵抗力。
