Kuo Ping-Chung, Li Yue-Chiun, Kusuma Anjar M, Tzen Jason T C, Hwang Tsong-Long, Ye Guan-Hong, Yang Mei-Lin, Wang Sheng-Yang
School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan.
Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan.
Antioxidants (Basel). 2021 Apr 13;10(4):598. doi: 10.3390/antiox10040598.
needle tea are very popular in Eastern countries such as Japan, Russia, Korea, and China. Pine needle tea is claimed to have significant anti-aging effects, but no clear evidence has supported this until now. In the present study, five undescribed compounds (-) as well as seventy-two known compounds were purified and characterized from the bioactive fraction of methanol extracts of needles. Most of the isolates were examined for their anti-inflammatory bioactivity by cellular neutrophil model and six compounds (, , , , , and ) exhibited a significant inhibition on superoxide anion generation and elastase release with IC values ranging from 3.3 ± 0.9 to 8.3 ± 0.8 μM. These anti-inflammatory ingredients were subjected to docking computing to evaluate their binding affinity on the ghrelin receptor, which played an important role in regulating metabolism, with anti-aging effects. Compounds , , and formed a stable complex with the ghrelin receptor via hydrogen bonds and different types of interactions. These results suggest the flavonoids are responsible for the potential anti-aging effects of pine needle tea.
针叶茶在日本、俄罗斯、韩国和中国等东方国家非常受欢迎。松针茶据称具有显著的抗衰老作用，但迄今为止尚无明确证据支持这一点。在本研究中，从针叶甲醇提取物的生物活性部分中纯化并鉴定了5种未描述的化合物以及72种已知化合物。通过细胞中性粒细胞模型对大多数分离物的抗炎生物活性进行了检测，6种化合物（、、、、和）对超氧阴离子生成和弹性蛋白酶释放表现出显著抑制作用，IC值范围为3.3±0.9至8.3±0.8μM。这些抗炎成分进行对接计算，以评估它们对胃饥饿素受体的结合亲和力，胃饥饿素受体在调节新陈代谢中起重要作用，具有抗衰老作用。化合物、和通过氢键和不同类型的相互作用与胃饥饿素受体形成稳定的复合物。这些结果表明黄酮类化合物是松针茶潜在抗衰老作用的原因。
