Numerical Modelling of the Optical Properties of Plasmonic and Latex Nanoparticles to Improve the Detection Limit of Immuno-Turbidimetric Assays.

Turbidimetric assays with latex nanoparticles are widely applied for the detection of biological analytes, because of their rapidity, low cost, reproducibility, and automatization. However, the detection limit can be lowered only at the price of a reduced dynamic range, due to the rapid saturation of the light scattering signal at high analyte concentration. Here, we use numerical calculations to investigate the possibility of increasing the performance of immuno-turbidimetric assays without compromising the measurement dynamic range, by combining plasmonic (gold, silver) and latex nanoparticles. Our modelling results show that plasmonic nanoparticles are compatible with a large signal change even when small aggregates are formed, i.e., at low analyte concentration. The working principle relies on the remarkable modification of the surface plasmon band when noble metal nanoparticles form oligomers, and also when latex particles are included in the aggregate. At high analyte concentration, when larger aggregates form, the latex particles can provide the required linear response of standard immuno-turbidimetric assays. Thus, the combination of the two components can be a successful strategy to improve the detection limit and the dynamic range, while maintaining all the advantages of the homogeneous immuno-turbidimetric assays.