用于评估精子DNA损伤的种间胞浆内单精子注射：技术报告
Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report.
作者信息
Rychtarova Jana, Langerova Alena, Fulka Helena, Loi Pasqualino, Benc Michal, Fulka Josef
机构信息
Institute of Animal Science, 104 00 Prague, Czech Republic.
GENNET, 170 00 Prague, Czech Republic.
出版信息
Animals (Basel). 2021 Apr 26;11(5):1250. doi: 10.3390/ani11051250.
Abstract
Xenogenic mammalian sperm heads injected into mouse ovulated oocytes decondense and form pronuclei in which sperm DNA parameters can be evaluated. We suggest that this approach can be used for the assessment of sperm DNA damage level and the evaluation of how certain sperm treatments (freezing, lyophilization, etc.) influence the quality of spermatozoa.
摘要
将异种哺乳动物的精子头部注射到小鼠排卵的卵母细胞中，精子头部会解聚并形成原核，在原核中可以评估精子的DNA参数。我们认为，这种方法可用于评估精子DNA损伤水平，以及评估某些精子处理方法（冷冻、冻干等）对精子质量的影响。
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