Department of General, Visceral and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Chronic Immune Reactions, German Rheumatism Research Center, Berlin, Germany.
Methods Mol Biol. 2021;2285:1-25. doi: 10.1007/978-1-0716-1311-5_1.
Isolation of cells from organs and tissues represents a challenge for many researchers. Cell yield, purity, and cell death are common problems associated with it, which greatly affect experimental results in terms of reproducibility and biological observations. Here, we describe state-of-the-art protocols of how to isolate CD4 T cells from both human and murine organs and tissues, reducing at minimum cell death, while increasing at the same time cell yield and purity.
从器官和组织中分离细胞对许多研究人员来说是一个挑战。细胞产量、纯度和细胞死亡是常见的问题，这极大地影响了实验结果的可重复性和生物学观察。在这里，我们描述了如何从人和鼠的器官和组织中分离 CD4 T 细胞的最新技术方案，在最小化细胞死亡的同时，提高细胞产量和纯度。
