Deutsches Rheuma-Forschungszentrum (DRFZ) Berlin, an Institute of the Leibniz Association, Berlin, Germany.
Maurice Müller Laboratories (Department of Biomedical Research), Universitätsklinik für Viszerale Chirurgie und Medizin Inselspital, University of Bern, Bern, Switzerland.
Methods Mol Biol. 2021;2285:349-373. doi: 10.1007/978-1-0716-1311-5_26.
Inflammatory bowel disease (IBD) is a group of severe chronic inflammatory conditions of the human gastrointestinal tract. Murine models of colitis have been invaluable tools to improve the understanding of IBD development and pathogenesis. While the disease etiology of IBD is complex and multifactorial, CD4 T helper cells have been shown to strongly contribute to the disease pathogenesis of IBD. Here, we present a detailed protocol of the preclinical model of T-cell transfer colitis, which can easily be utilized in the laboratory to study T helper cell functions in intestinal inflammation.
炎症性肠病（IBD）是一组严重的人类胃肠道慢性炎症性疾病。结肠炎的啮齿动物模型是提高对 IBD 发展和发病机制理解的宝贵工具。虽然 IBD 的疾病病因复杂且多因素，但已表明 CD4 T 辅助细胞强烈促成 IBD 的发病机制。在这里，我们提供了 T 细胞转移结肠炎临床前模型的详细方案，该方案可在实验室中轻松用于研究肠道炎症中辅助性 T 细胞的功能。
