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A Ca(2+) concentration of 1.5 mM, as present in IMDM but not in RPMI, is critical for maximal response of Th cells to PMA/ionomycin.

作者信息

Zimmermann Jakob, Radbruch Andreas, Chang Hyun-Dong

机构信息

Cell Biology Group, Deutsches Rheumaforschungszentrum, Berlin, Germany, a Leibniz Institute.

出版信息

Eur J Immunol. 2015 Apr;45(4):1270-3. doi: 10.1002/eji.201445247. Epub 2015 Feb 17.

DOI:10.1002/eji.201445247
PMID:25545753
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4407954/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e2/4407954/210925705d31/eji0045-1270-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e2/4407954/b5eca7af5c63/eji0045-1270-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e2/4407954/210925705d31/eji0045-1270-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e2/4407954/b5eca7af5c63/eji0045-1270-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e2/4407954/210925705d31/eji0045-1270-f2.jpg

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J Biol Chem. 2014 Sep 26;289(39):26752-26761. doi: 10.1074/jbc.M114.587865. Epub 2014 Jul 17.
2
An animated landscape representation of CD4+ T-cell differentiation, variability, and plasticity: insights into the behavior of populations versus cells.CD4+ T细胞分化、变异性和可塑性的动态景观呈现:对群体与细胞行为的见解
Eur J Immunol. 2014 Aug;44(8):2216-29. doi: 10.1002/eji.201444645.
3
Isolation of NK cells and NK-like cells from the intestinal lamina propria.
钙离子浓度会影响体外培养的小鼠和人淋巴细胞细胞因子的产生以及人巨噬细胞的极化。
PLoS One. 2023 Feb 24;18(2):e0282037. doi: 10.1371/journal.pone.0282037. eCollection 2023.
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).流式细胞术和细胞分选在免疫学研究中的应用指南(第三版)。
Eur J Immunol. 2021 Dec;51(12):2708-3145. doi: 10.1002/eji.202170126. Epub 2021 Dec 7.
5
Evaluation of Cytokine Production at the Single Cell Level by Flow Cytometry Upon Polyclonal Stimulation.采用流式细胞术对多克隆刺激后单细胞水平细胞因子产生的评估。
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