Department of Urology Surgery, Caoxian People's Hospital, Heze, China.
Eur Rev Med Pharmacol Sci. 2021 Apr;25(8):3211-3220. doi: 10.26355/eurrev_202104_25729.
The aim of this study was to investigate the role of long non-coding Opa-interacting protein 5 antisense RNA 1 (OIP5-AS1) in bladder cancer (BCa), and the mechanism of OIP5-AS1/microRNA-217 (miR-217)/metadherin (MTDH) in promoting the progression of BCa.
OIP5-AS1, miR-217 and MTDH expressions in BCa tissues and cells were detected by qRT-PCR or Western blot. CCK-8 and transwell assays were used to determine the proliferation and invasion of BCa cells. The correlation between OIP5-AS1 and miR-217, miR-217 and MTDH, and OIP5-AS1 and MTDH were studied by Luciferase reporter assay and Spearman correlation analysis. Statistical analysis of test data was performed using t-test.
OIP5-AS1 was upregulated in BCa tissues and cells, and OIP5-AS1 knockdown could inhibit the proliferation and invasion of BCa cells. MiR-217 was a direct-acting target of OIP5-AS1, and MTDH was a target of miR-217. OIP5-AS1 knockdown inhibits human BCa cell proliferation and invasion through miR-217/MTDH axis.
This study systematically explored the effect of OIP5-AS1 in human BCa. MiR-217/MTDH pathway mediated the promotion of OIP5-AS1 in BCa cells proliferation and invasion. OIP5-AS1, as an oncogene, could be used as a biomarker for the treatment of BCa.
本研究旨在探讨长链非编码 Opa 相互作用蛋白 5 反义 RNA 1（OIP5-AS1）在膀胱癌（BCa）中的作用，以及 OIP5-AS1/微小 RNA-217（miR-217）/间充质表皮转化因子（MTDH）在促进 BCa 进展中的机制。
通过 qRT-PCR 或 Western blot 检测 BCa 组织和细胞中的 OIP5-AS1、miR-217 和 MTDH 表达。CCK-8 和 Transwell 测定法用于测定 BCa 细胞的增殖和侵袭。通过荧光素酶报告基因测定和 Spearman 相关分析研究 OIP5-AS1 与 miR-217、miR-217 与 MTDH 以及 OIP5-AS1 与 MTDH 之间的相关性。采用 t 检验对检验数据进行统计学分析。
OIP5-AS1 在 BCa 组织和细胞中上调，OIP5-AS1 敲低可抑制 BCa 细胞的增殖和侵袭。miR-217 是 OIP5-AS1 的直接作用靶点，MTDH 是 miR-217 的靶点。OIP5-AS1 敲低通过 miR-217/MTDH 轴抑制人 BCa 细胞的增殖和侵袭。
本研究系统地探讨了 OIP5-AS1 在人 BCa 中的作用。miR-217/MTDH 通路介导了 OIP5-AS1 促进 BCa 细胞增殖和侵袭的作用。OIP5-AS1 作为一种癌基因，可作为 BCa 治疗的标志物。
