Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young, Dr. E., Los Angeles, CA, 90095, USA.
Angew Chem Int Ed Engl. 2021 Aug 2;60(32):17362-17367. doi: 10.1002/anie.202102413. Epub 2021 Jun 29.
The clinical utility of emulsions as delivery vehicles is hindered by a dependence on passive release. Stimuli-responsive emulsions overcome this limitation but rely on external triggers or are composed of nanoparticle-stabilized droplets that preclude sizes necessary for biomedical applications. Here, we employ cleavable poly(2-oxazoline) diblock copolymer surfactants to form perfluorocarbon (PFC) nanoemulsions that release cargo upon exposure to glutathione. These surfactants allow for the first example of redox-responsive nanoemulsions in cellulo. A noncovalent fluorous tagging strategy is leveraged to solubilize a GFP plasmid inside the PFC nanoemulsions, whereupon protein expression is achieved selectively when employing a stimuli-responsive surfactant. This work contributes a methodology for non-viral gene delivery and represents a general approach to nanoemulsions that respond to endogenous stimuli.
乳液作为药物载体的临床应用受到被动释放的限制。刺激响应型乳液克服了这一限制，但依赖于外部触发因素或由纳米颗粒稳定的液滴组成，这些液滴排除了生物医学应用所需的尺寸。在这里，我们使用可裂解的聚（2-恶唑啉）两亲嵌段共聚物表面活性剂来形成全氟碳（PFC）纳米乳液，在暴露于谷胱甘肽时释放货物。这些表面活性剂允许首次在细胞内实现氧化还原响应纳米乳液的实例。利用非共价氟标记策略将 GFP 质粒溶解在 PFC 纳米乳液中，然后在使用刺激响应表面活性剂时选择性地实现蛋白质表达。这项工作为非病毒基因传递提供了一种方法，代表了一种响应内源性刺激的纳米乳液的通用方法。
