尼古丁配方会影响蒸气自我给药小鼠模型中的强化相关行为。
Nicotine formulations impact reinforcement-related behaviors in a mouse model of vapor self-administration.
机构信息
Department of Biomedical Sciences, Joan C Edwards School of Medicine at Marshall University, 1700 3(rd) ave, Huntington, WV, 25703, USA.
出版信息
Drug Alcohol Depend. 2021 Jul 1;224:108732. doi: 10.1016/j.drugalcdep.2021.108732. Epub 2021 Apr 24.
Abstract
BACKGROUND
Electronic nicotine delivery systems (ENDS) differ from combustible cigarettes given that nicotine-salt or nicotine-freebase may be used depending on the product. We have investigated how nicotine-salt and freebase formulations alter e-Vape® self-administration (EVSA) behavior and plasma cotinine levels in male and female mice.
METHODS
Adult C57/BL6 J mice were used in EVSA and assigned vaping e-liquids (50:50 PGVG, 6 mg/mL nicotine-freebase, or 6 mg/mL nicotine-salt). Mice were escalated on a fixed ratio 1 (FR1) schedule in daily 2 h sessions and then transitioned to a FR3 to examine reinforcement-related behaviors.
RESULTS
Here we observed that mice assigned nicotine-salt exhibited increased EVSA on a FR3 schedule compared to nicotine-freebase. Additionally, mice assigned nicotine-salt exhibited higher plasma cotinine concentrations following delivery-controlled passive-inhalation sessions.
CONCLUSIONS
These data provide evidence nicotine-salt formulations may contribute to greater reinforcement-related behavior and highlight the need for further investigations regarding nicotine formulation in ENDS.
摘要
背景
电子尼古丁传送系统（ENDS）与可燃香烟不同，因为尼古丁盐或游离碱的使用取决于产品。我们研究了尼古丁盐和游离碱配方如何改变雄性和雌性小鼠的电子烟自我给药（EVSA）行为和血浆可替宁水平。
方法
成年 C57/BL6 J 小鼠用于 EVSA，并分配电子烟液（50:50 PGVG、6mg/mL 游离碱尼古丁或 6mg/mL 尼古丁盐）。小鼠在每日 2 小时的固定比率 1（FR1）时间表上逐步递增，然后过渡到 FR3，以检查与强化相关的行为。
结果
在这里，我们观察到分配尼古丁盐的小鼠在 FR3 时间表上的 EVSA 增加，与游离碱尼古丁相比。此外，在经过输送控制的被动吸入后，分配尼古丁盐的小鼠表现出更高的血浆可替宁浓度。
结论
这些数据提供了证据，表明尼古丁盐配方可能会导致更大的与强化相关的行为，并强调了在 ENDS 中进一步研究尼古丁配方的必要性。
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