Department of Burn and Plastic Surgery, The 980st Hospital of the PLA Joint Logistics Support Force, Shijiazhuang, China.
Department of Plastic and Reconstructive Surgery, The First Medical Center, Chinese PLA General Hospital, Beijing, China.
FEBS Open Bio. 2021 Jun;11(6):1673-1684. doi: 10.1002/2211-5463.13167. Epub 2021 May 12.
Hypertrophic scarring (HS) is a dermal fibroproliferative disorder characterized by excessive deposition of collagen and other extracellular matrix components. The aim of this study is to explore crucial long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) associated with HS and provide a better understanding of the molecular mechanism of HS. To investigate the lncRNA, circRNA and mRNA expression profiles, we performed RNA sequencing of human HS and normal skin tissues. After the identification of differentially expressed mRNAs (DEmRNAs), lncRNAs (DElncRNAs) and circRNAs (DEcircRNAs), we performed functional enrichment of DEmRNAs. Further on, we constructed DElncRNA/DEcircRNA-DEmRNA coexpression networks and competing endogenous RNA regulatory networks, and performed functional analyses of the DEmRNAs in the constructed networks. In total, 487 DEmRNAs, 92 DElncRNAs and 17 DEcircRNAs were identified. DEmRNAs were significantly enriched in processes such as collagen fibril organization, extracellular matrix-receptor interaction and the phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway. In addition, we detected 580 DElncRNA-DEmRNA and 505 DEcircRNA-DEmRNA coexpression pairs. The competing endogenous RNA network contained 18 circRNA-microRNA (miRNA) pairs, 18 lncRNA-miRNA pairs and 409 miRNA-mRNA pairs, including 10 circRNAs, 5 lncRNAs, 15 miRNAs and 160 mRNAs. We concluded that MIR503HG/hsa-miR-204-3p/ACAN, MIR503HG/hsa-miR-431-5p/TNFRSF9, MEG3/hsa-miR-6884-5p/ADAMTS14, AC000035.1-ADAMTS14 and hsa_circ_0069865-COMP/ADAM12 interaction pairs may play a central role in HS.
增生性瘢痕（HS）是一种以胶原和其他细胞外基质成分过度沉积为特征的真皮纤维增生性疾病。本研究旨在探讨与 HS 相关的关键长链非编码 RNA（lncRNA）和环状 RNA（circRNA），为 HS 的分子机制提供更好的理解。为了研究 lncRNA、circRNA 和 mRNA 的表达谱，我们对人 HS 和正常皮肤组织进行了 RNA 测序。在鉴定差异表达的 mRNAs（DEmRNAs）、lncRNAs（DElncRNAs）和 circRNAs（DEcircRNAs）后，我们对 DEmRNAs 进行了功能富集分析。进一步构建了 DElncRNA/DEcircRNA-DEmRNA 共表达网络和竞争内源性 RNA 调控网络，并对构建网络中的 DEmRNAs 进行了功能分析。总共鉴定出 487 个 DEmRNAs、92 个 DElncRNAs 和 17 个 DEcircRNAs。DEmRNAs 显著富集于胶原纤维组织、细胞外基质-受体相互作用和磷脂酰肌醇 3-激酶（PI3K）-Akt 信号通路等过程中。此外，我们检测到 580 个 DElncRNA-DEmRNA 和 505 个 DEcircRNA-DEmRNA 共表达对。竞争内源性 RNA 网络包含 18 个 circRNA-miRNA（miRNA）对、18 个 lncRNA-miRNA 对和 409 个 miRNA-mRNA 对，包括 10 个 circRNAs、5 个 lncRNAs、15 个 miRNAs 和 160 个 mRNAs。我们得出结论，MIR503HG/hsa-miR-204-3p/ACAN、MIR503HG/hsa-miR-431-5p/TNFRSF9、MEG3/hsa-miR-6884-5p/ADAMTS14、AC000035.1-ADAMTS14 和 hsa_circ_0069865-COMP/ADAM12 相互作用对可能在 HS 中发挥核心作用。
