Department of Chemistry, Lehigh University, 6 E. Packer Ave. Bethlehem, PA 18015, USA.
Department of Chemistry, Lehigh University, 6 E. Packer Ave. Bethlehem, PA 18015, USA.
Biochim Biophys Acta Biomembr. 2021 Aug 1;1863(8):183624. doi: 10.1016/j.bbamem.2021.183624. Epub 2021 Apr 30.
Lipid droplets also known as oil bodies are found in a variety of organisms and function as stores of high-energy metabolites. Recently, there has been interest in using lipid droplets for protein production and drug delivery. Artificial lipid droplets have been previously prepared, but their short lifetime in solution and inhomogeneity has severely limited their applicability. Herein we report an improved methodology for the production of synthetic lipid droplets that overcomes the aforementioned limitations. These advancements include: 1) development of a methodology for the expression and purification of high-levels of oleosin, a crucial lipid droplet component, 2) preparation of neutrally-buoyant synthetic lipid droplets, and 3) production of synthetic lipid droplets of a specific size. Together, these important enhancements will facilitate the advancement of lipid droplet science and its application in biotechnology.
脂滴也被称为油体，存在于各种生物体中，作为高能代谢物的储存库。最近，人们对利用脂滴进行蛋白质生产和药物输送产生了兴趣。已经有先前制备人工脂滴的方法，但它们在溶液中的短寿命和非均相性严重限制了它们的适用性。在此，我们报告了一种改进的生产合成脂滴的方法，该方法克服了上述限制。这些进展包括：1）开发了一种表达和纯化油蛋白（一种关键的脂滴成分）的高水平的方法，2）制备中性浮力的合成脂滴，和 3）生产特定大小的合成脂滴。这些重要的改进将促进脂滴科学的发展及其在生物技术中的应用。
