Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China.
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China.
J Nutr Biochem. 2021 Aug;94:108750. doi: 10.1016/j.jnutbio.2021.108750. Epub 2021 Apr 29.
Lycopene has a wide range of biological functions, especially its antioxidant capacity. However, effects of lycopene on muscle fatigue resistant and muscle fiber type conversion are unknown. In this study, we found that lycopene significantly prolonged the swimming time to exhaustion in mice. We also showed that lycopene increased the proportion of slow-twitch muscle fiber by promoting muscle fiber type conversion from fast-twitch to slow-twitch in mice and in C2C12 myotubes. The AMP-activated protein kinase (AMPK) signaling was activated by lycopene. AMPK upstream and downstream regulators including nuclear respiratory factor 1, calcium calmodulin-dependent protein kinase kinase-β, sirtuin 1 and peroxisome proliferator activated receptor-γ coactivator-1ɑ were also increased by lycopene. AMPK inhibitor compound C markedly attenuated the lycopene-induced skeletal muscle fiber type conversion in C2C12 myotubes. Taken together, we provided the first evidence that lycopene increases the proportion of slow-twitch muscle fiber through AMPK signaling pathway to improve fatigue resistant of skeletal muscle.
番茄红素具有广泛的生物学功能，尤其是其抗氧化能力。然而，番茄红素对肌肉疲劳抗性和肌肉纤维类型转换的影响尚不清楚。在本研究中，我们发现番茄红素可显著延长小鼠力竭游泳时间。我们还表明，番茄红素通过促进肌肉纤维从快肌向慢肌的类型转换，增加了慢肌纤维的比例，在小鼠和 C2C12 肌管中也是如此。番茄红素激活了 AMP 激活的蛋白激酶（AMPK）信号通路。番茄红素还增加了 AMPK 的上游和下游调节因子，包括核呼吸因子 1、钙调蛋白依赖性蛋白激酶激酶-β、沉默调节蛋白 1 和过氧化物酶体增殖物激活受体-γ共激活因子-1α。AMPK 抑制剂化合物 C 明显减弱了番茄红素诱导的 C2C12 肌管中的骨骼肌纤维类型转换。综上所述，我们首次提供了证据表明，番茄红素通过 AMPK 信号通路增加慢肌纤维的比例，从而提高骨骼肌的抗疲劳能力。
