Department of Bio-nanotechnology, Gachon University, Gyeonggi-Do, Republic of Korea.
Department of Chemical and Biological Engineering, Gachon University, Gyeonggi-Do, Republic of Korea.
Colloids Surf B Biointerfaces. 2021 Aug;204:111773. doi: 10.1016/j.colsurfb.2021.111773. Epub 2021 Apr 19.
Ruthenium complexes have increased the scope for improvement in current cancer treatment by replacing platinum-based drugs. However, to reduce metal-associated toxicity, a biocompatible flavonoid, such as curcumin, is indispensable, as it offers uncompensated therapeutic benefits through formation of complexes. In this study, we synthesized metal-based flavonoid complexes using ruthenium(II) and curcumin by adopting a convenient reflux reaction, represented as Ru-Cur complexes. These complexes were thoroughly characterized using H, C NMR, XPS, FT-IR, and UV-vis spectroscopy. As curcumin is sparingly soluble in water and has poor chemical stability, we loaded Ru-Cur complexes into liposomes and further formed nanoparticles (NPs) using the thin layer evaporation method. These were named Ru-Cur loaded liposome nanoparticles (RCLNPs). The effects of RCLNPs on cell proliferation was investigated using human cervical cancer cell lines (HeLa). These RCLNPs exhibited significant cytotoxicity in HeLa cells. The anticancer properties of RCLNPs were studied using reactive oxygen species (ROS), LDH, and MTT assays as well as live-dead staining. Nuclear damage studies of RCLNPs were performed in HeLa cells using the Hoechst staining assay.
钌配合物通过取代基于铂的药物，增加了当前癌症治疗的改进范围。然而，为了降低金属相关的毒性，必需使用生物相容性的类黄酮，如姜黄素，因为它通过形成配合物提供了无补偿的治疗益处。在这项研究中，我们通过采用方便的回流反应，使用钌（II）和姜黄素合成了金属类黄酮配合物，表示为 Ru-Cur 配合物。这些配合物通过 H、C NMR、XPS、FT-IR 和 UV-vis 光谱进行了彻底的表征。由于姜黄素在水中的溶解度低且化学稳定性差，我们将 Ru-Cur 配合物载入脂质体中，并进一步使用薄层蒸发法形成纳米颗粒（NPs）。这些被命名为 Ru-Cur 负载脂质体纳米颗粒（RCLNPs）。使用人宫颈癌细胞系（HeLa）研究了 RCLNPs 对细胞增殖的影响。这些 RCLNPs 在 HeLa 细胞中表现出显著的细胞毒性。使用活性氧（ROS）、LDH 和 MTT 测定以及死活染色研究了 RCLNPs 的抗癌特性。使用 Hoechst 染色测定法在 HeLa 细胞中进行了 RCLNPs 的核损伤研究。
