Zhang Xing, He Hui, Xiang Jiqian, Li Bin, Zhao Mengge, Hou Tao
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Enshi Tujia & Miao Autonomous Prefecture Academy of Agricultural Sciences, Enshi 445000, China.
Food Chem. 2021 Oct 1;358:129888. doi: 10.1016/j.foodchem.2021.129888. Epub 2021 Apr 20.
Present study aimed to prepare and identify antioxidative peptides from selenium-containing soybeans, and to investigate their bioavailability and protective effects against oxidative stress-related diseases. Selenium-containing soybean antioxidative peptides (Mw < 1 kDa, SePPs) hydrolyzed by Neutrase and Alcalase reached the highest cellular antioxidant activity (EC value 320.5 ± 39.71 μg/L). SePPs could be efficiently absorbed through Caco-2 monolayer, and then significantly reverse the tumor necrosis factor-α (TNF-α)-induced inflammatory cytokine, phosphorylated c-Jun N-terminal kinases (p-JNK) and nuclear factor-kappa B (NF-κB) levels in EA. hy926 cells (p < 0.05). d-galactose-induced aging mice model showed that liver superoxidase dismutase (SOD) and glutathione peroxidase-1 (GPx-1) were enhanced, while aspartate aminotransferase (AST), alanine aminotransferase (ALT) and NF-κB were decreased by SePPs significantly (p < 0.05). SePPs could inhibit brain oxidative stress via regulating MAPK/NF-κB pathway. Comparing with NaSeO, selenomethionine (SeMet) and selenium-free peptides, SePPs was found to present synergistic effects of selenium and peptides in antioxidant activity.
本研究旨在从含硒大豆中制备并鉴定抗氧化肽，并研究其生物利用度以及对氧化应激相关疾病的保护作用。经中性蛋白酶和碱性蛋白酶水解得到的含硒大豆抗氧化肽（分子量<1 kDa，SePPs）具有最高的细胞抗氧化活性（EC值为320.5±39.71 μg/L）。SePPs能够通过Caco-2单层细胞有效吸收，进而显著逆转肿瘤坏死因子-α（TNF-α）诱导的EA.hy926细胞中炎性细胞因子、磷酸化c-Jun氨基末端激酶（p-JNK）和核因子-κB（NF-κB）的水平（p<0.05）。d-半乳糖诱导的衰老小鼠模型显示，SePPs可显著提高肝脏超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶-1（GPx-1）的水平，同时降低天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）和NF-κB的水平（p<0.05）。SePPs可通过调节MAPK/NF-κB途径抑制脑氧化应激。与亚硒酸钠、硒代蛋氨酸（SeMet）和无硒肽相比，SePPs在抗氧化活性方面呈现出硒和肽的协同作用。
