Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Universidad Nacional de Rosario, Suipacha 531, S2002LRK, Rosario, Santa Fe, Argentina.
YPF-Tecnología, Av. del Petróleo Arg. S/N, 1923, Berisso, Argentina.
Planta. 2021 May 2;253(5):115. doi: 10.1007/s00425-021-03633-0.
Trichoderma activates plant proteins to counteract Fusarium infection. Comparison between proteomic and transcriptomic data suggests differential response regulation. Proteins from the phenylpropanoid pathway are activated to quickly respond to pathogen attack. Trichoderma species can stimulate local and distant immune responses in colonized plant tissues to prevent future pathogenic attacks. Priming of plant defenses is characterized by changes in transcriptional, metabolic, and epigenetic states after stimulus perception. We have previously investigated transcriptional reprogramming in silk tissues from maize plants inoculated with Trichoderma atroviride and challenged with Fusarium verticillioides (Agostini et al., Mol Plant-Microbe In 32:95-106, 2019). To better understand the molecular changes induced by T. atroviride in maize, a proteomic approach was conducted in this instance. Several proteins belonging to different metabolic categories were detected as priming-involved proteins. However, we detected a very low correlation with those priming-modulated transcripts suggesting the importance of regulatory events a posteriori of the transcriptional process to accomplish the final goal of blocking pathogen entry. Specifically, we focused on the phenylpropanoid pathway, since we detected several proteins that are upregulated in the priming state and might explain cell wall reinforcement as well as the increase in flavonoid and lignin content in maize silks after activation of induced systemic resistance.
木霉激活植物蛋白以抵抗镰刀菌感染。蛋白质组学和转录组学数据的比较表明了差异调节反应。苯丙烷途径的蛋白质被激活以快速响应病原体攻击。木霉物种可以在定植植物组织中刺激局部和远距离的免疫反应，以防止未来的病原体攻击。植物防御的启动特征是在刺激感知后转录、代谢和表观遗传状态的变化。我们之前研究了用木霉菌属（ Trichoderma ）接种的玉米丝组织中的转录重编程，并用镰刀菌属（ Fusarium verticillioides ）（ Agostini 等人， Mol Plant-Microbe In 32:95-106, 2019）进行了挑战。为了更好地理解木霉菌属（ Trichoderma ）在玉米中诱导的分子变化，在这种情况下采用了蛋白质组学方法。检测到属于不同代谢类别的几种蛋白质作为参与启动的蛋白质。然而，我们检测到与那些启动调节转录本的相关性非常低，这表明在转录过程之后的调控事件对于完成阻止病原体进入的最终目标非常重要。具体来说，我们专注于苯丙烷途径，因为我们检测到几种在启动状态下上调的蛋白质，这些蛋白质可能解释了细胞壁的增强，以及在诱导系统抗性激活后玉米丝中类黄酮和木质素含量的增加。
