Nawrocka Justyna, Gromek Aleksandra, Małolepsza Urszula
Department of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Front Plant Sci. 2019 Apr 17;10:421. doi: 10.3389/fpls.2019.00421. eCollection 2019.
In the present study, TRS25 is presented as a biological control agent, which significantly limits the development of infection and reduces the disease caused by the pathogenic fungus in cucumber plants ( L.). The systemic disease suppression is related to oxidative, signaling, and biochemical changes, that are triggered in response to a pathogen. Induction of systemic defense in cucumber by TRS25 greatly depends on the accumulation of signaling molecules including hydrogen peroxide (HO) and nitric oxide (NO) as well as salicylic acid (SA) and its derivatives including methyl salicylate (MeSA) and octyl salicylate (OSA). The study established that NO was accumulated in leaves and shoots of the cucumber plants, especially those pretreated with and inoculated with , where the compound was accumulated mainly in the cells localized in the vascular bundles and in epidermal tissues. We suggest, for the first time, that in the plants pretreated with TRS25, the accumulation of HO and NO may be related to catalase (CAT) and S-nitrosoglutathione reductase (GSNOR) activity decrease. On the other hand, excessive accumulation of NO and SA may be controlled by forming their inactive forms, S-nitrosothiols (SNO) and salicylic acid glucosylated conjugates (SAGC), respectively. The obtained results suggest that the mentioned molecules may be an important component of the complex signaling network activated by TRS25, which is positively involved in systemic defense responses of cucumber plants against .
在本研究中，TRS25作为一种生物防治剂被提出，它能显著限制黄瓜植株（L.）中感染的发展并减少由致病真菌引起的病害。系统性病害抑制与病原体引发的氧化、信号传导和生化变化有关。TRS25诱导黄瓜的系统性防御很大程度上取决于信号分子的积累，包括过氧化氢（HO）、一氧化氮（NO）以及水杨酸（SA）及其衍生物，如甲基水杨酸酯（MeSA）和辛基水杨酸酯（OSA）。该研究证实，NO在黄瓜植株的叶片和茎中积累，尤其是那些用 预处理并接种 的植株，该化合物主要积累在维管束和表皮组织中的细胞中。我们首次提出，在用TRS25预处理的植株中，HO和NO的积累可能与过氧化氢酶（CAT）和S-亚硝基谷胱甘肽还原酶（GSNOR）活性降低有关。另一方面，NO和SA的过量积累可能分别通过形成它们的无活性形式，即S-亚硝基硫醇（SNO）和水杨酸糖基化共轭物（SAGC）来控制。所得结果表明，上述分子可能是由TRS25激活的复杂信号网络的重要组成部分，该网络积极参与黄瓜植株对 的系统性防御反应。
