Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China.
College of Natural Resources and Life Science, Dong-A University, Busan, Republic of Korea.
Pest Manag Sci. 2021 Sep;77(9):4159-4167. doi: 10.1002/ps.6453. Epub 2021 May 14.
Uridine diphosphate-glycosyltransferases (UGTs) are phase II metabolic enzymes involved in metabolism of toxins and resistance to insecticides in insect pests. Reactive oxygen species (ROS) induced by xenobiotics are important for activation of detoxification pathways. However, relationships between ROS and UGTs involved in toxin metabolism and insecticide resistance remain unclear.
Here, involvement of dual oxidase (Duox)-dependent ROS in regulating UGT expression-mediated insecticide resistance in the brown planthopper (Nilaparvata lugens) was investigated. The overexpression of NlUGT386F2 contributed to the resistance of N. lugens to clothianidin. Furthermore, the ROS inhibitor (N-acetylcysteine) significantly reduced the expression of NlUGT386F2 and increased the susceptibility of N. lugens to clothianidin. Silencing the ROS producer Duox significantly increased the susceptibility of N. lugens to clothianidin through the down-regulation of NlUGT386F2 expression.
NlDuox-dependent ROS regulates NlUGT386F2 expression-mediated clothianidin resistance in brown planthopper. These observations further our understanding of the metabolism of toxins and of insecticide-resistance in insect pests.
尿苷二磷酸-糖基转移酶（UGTs）是参与毒素代谢和昆虫抗药性的 II 相代谢酶。外源性化学物质诱导的活性氧（ROS）对于解毒途径的激活很重要。然而，ROS 与参与毒素代谢和抗药性的 UGTs 之间的关系仍不清楚。
本研究探讨了双氧化酶（Duox）依赖性 ROS 在调节褐飞虱（Nilaparvata lugens）UGT 表达介导的杀虫剂抗性中的作用。NlUGT386F2 的过表达有助于褐飞虱对噻虫嗪的抗性。此外，ROS 抑制剂（N-乙酰半胱氨酸）显著降低了 NlUGT386F2 的表达，增加了褐飞虱对噻虫嗪的敏感性。ROS 产生器 Duox 的沉默通过下调 NlUGT386F2 的表达显著增加了褐飞虱对噻虫嗪的敏感性。
NlDuox 依赖性 ROS 调节褐飞虱中 NlUGT386F2 表达介导的噻虫嗪抗性。这些观察结果进一步加深了我们对昆虫毒素代谢和抗药性的理解。
