Meng Jiali, Mäkelä Miia R, de Vries Ronald P
Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Utrecht, The Netherlands.
Department of Microbiology, University of Helsinki, Helsinki, Finland.
Adv Appl Microbiol. 2021;114:73-109. doi: 10.1016/bs.aambs.2020.09.001. Epub 2020 Oct 17.
Lignocellulosic biomass is an abundant and renewable resource, and its utilization has become the focus of research and biotechnology applications as a very promising raw material for the production of value-added compounds. Filamentous fungi play an important role in the production of various lignocellulolytic enzymes, while some of them have also been used for the production of important metabolites. However, wild type strains have limited efficiency in enzyme production or metabolic conversion, and therefore many efforts have been made to engineer improved strains. Examples of this are the manipulation of transcriptional regulators and/or promoters of enzyme-encoding genes to increase gene expression, and protein engineering to improve the biochemical characteristics of specific enzymes. This review provides and overview of the applications of filamentous fungi in lignocellulosic biomass based processes and the development and current status of various molecular engineering strategies to improve these processes.
木质纤维素生物质是一种丰富的可再生资源，作为生产增值化合物的极具前景的原材料，其利用已成为研究和生物技术应用的焦点。丝状真菌在各种木质纤维素分解酶的生产中发挥着重要作用，其中一些还被用于生产重要的代谢产物。然而，野生型菌株在酶生产或代谢转化方面效率有限，因此人们已做出诸多努力来构建改良菌株。例如，操纵转录调节因子和/或酶编码基因的启动子以增加基因表达，以及进行蛋白质工程以改善特定酶的生化特性。本综述概述了丝状真菌在基于木质纤维素生物质的工艺中的应用，以及用于改进这些工艺的各种分子工程策略的发展和现状。
