Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium; Center for Advanced Process Technology and Urban Resource Efficiency (CAPTURE), Frieda Saeysstraat, 9052 Ghent, Belgium.
Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), P.O. Box 5003, 1432 Ås, Norway; Protein Chemistry and Enzyme Technology Section, DTU Bioengineering, Department of Biotechnology and Biomedicine, Technical University of Denmark, 2800 Kgs Lyngby, Denmark.
Bioresour Technol. 2022 Jan;344(Pt A):126200. doi: 10.1016/j.biortech.2021.126200. Epub 2021 Oct 26.
Biomass represents an abundant and inexpensive source of sugars and aromatic compounds that can be used as raw materials for conversion into value-added bioproducts. Filamentous fungi are sources of plant cell wall degrading enzymes in nature. Understanding the interactions between enzymes is crucial for optimizing biomass degradation processes. Herein, the concept of the interactome is presented as a holistic approach that depicts the interactions among enzymes, substrates, metabolites, and inhibitors. The interactome encompasses several stages of biomass degradation, starting with the sensing of the substrate and the subsequent synthesis of hydrolytic and oxidative enzymes (fungus-substrate interaction). Enzyme-enzyme interactions are exemplified in the complex processes of lignocellulosic biomass degradation. The enzyme-substrate-metabolite-inhibitor interaction also provides a better understanding of biomass conversion, allowing bioproduct production from recalcitrant agro-industrial residues, thus bringing greater value to residual biomass. Finally, technological applications are presented for optimizing the interactome at various levels.
生物量是一种丰富且廉价的糖和芳香族化合物来源，可以用作将其转化为高附加值生物制品的原料。丝状真菌是自然界中植物细胞壁降解酶的来源。了解酶之间的相互作用对于优化生物质降解过程至关重要。本文提出了互作组学的概念，它是一种整体方法，描述了酶、底物、代谢物和抑制剂之间的相互作用。互作组学涵盖了生物质降解的几个阶段，从底物的感应开始，随后合成水解和氧化酶（真菌-底物相互作用）。在木质纤维素生物质降解的复杂过程中，酶-酶相互作用得到了例证。酶-底物-代谢物-抑制剂相互作用也为生物质转化提供了更好的理解，从而可以从难处理的农业工业残留物中生产生物制品，从而为剩余生物质带来更大的价值。最后，提出了在各个层面优化互作组学的技术应用。
