Nautiyal Vipin, Dubey R C
Department of Botany and Microbiology, Gurukula Kangri (Deemed to be University), Haridwar - 249404, Uttarakhand, India.
Saudi J Biol Sci. 2021 Apr;28(4):2432-2437. doi: 10.1016/j.sjbs.2021.01.041. Epub 2021 Feb 1.
The main emphasis of this study was to identify the bioactive compounds responsible for antibacterial activity of cow urine isolated by thin layer chromatography. The most effective bioactive fraction was analysed by FT-IR and GC-MS analyses. Among the four major fractions (EW1, EW2, CA1 and CA2) obtained by TLC profiling, EW1 was found most active against bacterial strains viz., (MTCC657), (MTCC7443), (MTCC424), (MTCC432) and (MTCC733). However, (MTCC118), was found resistant to all the fractions. In FT-IR spectroscopy, functional groups like alcohol, amide, alkene, alkyl halide, polysulfide and phosphate ions were identified. The GC-MS analysis of EW1 fraction exhibited the presence of 12 compounds, of which 1-heneicosanol was found as the major compound. These compounds might be responsible synergistically or individually for antibacterial activity of cow urine. Nine elements namely sodium (Na), calcium (Ca), chromium (Cr), iron (Fe), magnesium (Mg), aluminium (Al), potassium (K) and zinc (Zn), Gold (Au) were measured by ICP-MS analysis.
本研究的主要重点是通过薄层色谱法鉴定牛尿抗菌活性的生物活性化合物。通过傅里叶变换红外光谱（FT-IR）和气相色谱-质谱联用（GC-MS）分析对最有效的生物活性部分进行了分析。在通过薄层色谱分析获得的四个主要部分（EW1、EW2、CA1和CA2）中，发现EW1对细菌菌株即（MTCC657）、（MTCC7443）、（MTCC424）、（MTCC432）和（MTCC733）最具活性。然而，（MTCC118）对所有部分均具有抗性。在傅里叶变换红外光谱分析中，鉴定出了醇、酰胺、烯烃、卤代烃、多硫化物和磷酸根离子等官能团。EW1部分的气相色谱-质谱联用分析显示存在12种化合物，其中1-二十一烷醇被发现是主要化合物。这些化合物可能协同或单独对牛尿的抗菌活性负责。通过电感耦合等离子体质谱（ICP-MS）分析测量了钠（Na）、钙（Ca）、铬（Cr）、铁（Fe）、镁（Mg）、铝（Al）、钾（K）、锌（Zn）、金（Au）九种元素。
