Kharat Avinash, Sakhare Swapnali, Sanap Avinash, Potdar Pranjali, Kheur Supriya, Bhonde Ramesh
Regenerative Medicine Laboratory, Dr. D. Y. Patil Dental College and Hospital, Dr. D.Y.Patil Vidyapeeth, Pimpri, Pune, India.
Regenerative Medicine Laboratory, Dr. D. Y. Patil Dental College and Hospital, Dr. D.Y.Patil Vidyapeeth, Pimpri, Pune, India.
J Ayurveda Integr Med. 2025 Jul 2;16(4):101194. doi: 10.1016/j.jaim.2025.101194.
Cow urine has been utilized in traditional Indian medicine for the treatment of various ailments such as wounds, oxidative stress, and the management of various metabolic disorders with limited toxicity. However, there is a scarcity of scientific evidence confirming its exact mode of action.
The aim of the study is to examine the mode of action of cow urine, also known as "Gomutra", focusing on its effect on human dental pulp stem cells (DPSCs) and chick yolk sac membrane (YSM) models.
DPSCs were isolated and characterised using a flow analyser, and used to assess the cytotoxicity, osteogenic and chondrogenic differentiation potential, anti-aging activity, anti-oxidative nature. Angiogenic potential was evaluated by YSM model.
DPSCs showed fibroblast-like morphology and expressed mesenhymal stem cell markers viz., CD73, CD90, and CD105, and lacked expression of CD34, CD45 and HLA-DR. Our findings indicated that cow urine showed no toxicity at 1, 2, 4, 5, 10, 20 and 25% concentrations and promoted osteogenic and chondrogenic differentiation of DPSCs compared to standard controls. Additionally, cow urine effectively reduced oxidative stress and delayed cellular senescence in DPSCs, suggesting its anti-aging potential. Further, the study evidenced cow urine's angiogenic activity through YSM model.
This study provides substantial evidence for the non-toxic nature of cow urine. Our data demonstrates bone and cartilage regeneration, ant-aging, angiogenic and anti-oxidative nature of cow urine indicating its therapeutic value.
在印度传统医学中，牛尿已被用于治疗各种疾病，如伤口、氧化应激以及各种代谢紊乱的管理，且毒性有限。然而，缺乏科学证据证实其确切作用方式。
本研究旨在探讨牛尿（又称“Gomutra”）的作用方式，重点关注其对人牙髓干细胞（DPSCs）和鸡卵黄囊膜（YSM）模型的影响。
使用流式分析仪分离并鉴定DPSCs，用于评估细胞毒性、成骨和成软骨分化潜能、抗衰老活性、抗氧化性质。通过YSM模型评估血管生成潜能。
DPSCs呈现成纤维细胞样形态，表达间充质干细胞标志物，即CD73、CD90和CD105，且不表达CD34、CD45和HLA - DR。我们的研究结果表明，牛尿在1%、2%、4%、5%、10%、20%和25%浓度下无毒性，与标准对照相比，可促进DPSCs的成骨和成软骨分化。此外，牛尿有效降低了DPSCs中的氧化应激并延缓了细胞衰老，表明其具有抗衰老潜能。此外，该研究通过YSM模型证明了牛尿的血管生成活性。
本研究为牛尿的无毒性质提供了大量证据。我们的数据表明牛尿具有骨和软骨再生、抗衰老、血管生成和抗氧化性质，表明其具有治疗价值。
