Li Mingjiu, Guo Ganggang, Pidon Hélène, Melzer Michael, Prina Alberto R, Börner Thomas, Stein Nils
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Seeland, Germany.
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Plant Sci. 2021 Apr 16;12:664085. doi: 10.3389/fpls.2021.664085. eCollection 2021.
Implementation of next-generation sequencing in forward genetic screens greatly accelerated gene discovery in species with larger genomes, including many crop plants. In barley, extensive mutant collections are available, however, the causative mutations for many of the genes remains largely unknown. Here we demonstrate how a combination of low-resolution genetic mapping, whole-genome resequencing and comparative functional analyses provides a promising path toward candidate identification of genes involved in plastid biology and/or photosynthesis, even if genes are located in recombination poor regions of the genome. As a proof of concept, we simulated the prediction of a candidate gene for the recently cloned variegation mutant () and adopted the approach for suggesting as candidate gene for the yellow-green variegation mutant .
在正向遗传筛选中实施新一代测序极大地加速了包括许多农作物在内的大基因组物种中的基因发现。在大麦中，有大量的突变体库，然而，许多基因的致病突变在很大程度上仍然未知。在这里，我们展示了低分辨率遗传图谱、全基因组重测序和比较功能分析的组合如何为鉴定参与质体生物学和/或光合作用的基因的候选基因提供了一条有前景的途径，即使基因位于基因组中重组率低的区域。作为概念验证，我们模拟了对最近克隆的杂色突变体（）候选基因的预测，并采用该方法将 作为黄绿色杂色突变体的候选基因。
