Ghasemi Maryam, Vahedi Larijani Laleh, Yazdani-Charati Jamshid, Kamali Hakim Elham
Department of Pathology, Immunogenetics Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Biostatistics, Health Sciences Research Center, School of Health, Mazandaran University of Medical Sciences, Sari, Iran.
Iran J Pathol. 2021 Spring;16(2):195-204. doi: 10.30699/IJP.2021.121140.2318. Epub 2021 Mar 2.
BACKGROUND & OBJECTIVE: Galectin-8 has relationships with cell growth and metastasis of some cancers. Due to controversy in the clinical significance of this protein in the cancer process, we investigated its roles in the development of head and neck squamous cell carcinoma.
This study was performed on 93 samples of patients with Squamous Cell Carcinoma or dysplasia of the head and neck, who underwent biopsy or surgery from 2015 till 2017 in Boo-Ali SINA hospital of Sari, Iran. The relevant paraffin embedded tissue blocks were obtained from archive of pathology and evaluated for galectin-8 by immunohistochemistry. The association between expression of galactin-8 and age, sex, location and stage of disease were assessed. To compare expression rate between the groups, Mc-Nemar, Chi-square and Fisher's exact tests were used. The P-value<0.05 was considered significant.
Strong cytoplasmic and nuclear galactin-8 staining was observed in 97.6% cases of normal tissues while 77% of dysplastic lesions and 69% of the cancers revealed negative immunoreactivity. The intensity of expression in dysplastic and malignant tissues was significantly reduced compared with normal tissues (=0.0001). The expression of galectin-8 did not correlate with stage (=0.303), lymph node involvement (=0.326), tumor grade (=0.769), distant metastasis (=0.748), and age (=0.574).
We observed that the expression of galectin-8 in dysplastic and malignant squamous epithelium significantly reduced compared with the normal counterpart of them in the head and neck. It may contribute to malignant transformation of head and neck squamous cells.
半乳糖凝集素-8与某些癌症的细胞生长和转移有关。由于该蛋白在癌症进程中的临床意义存在争议，我们研究了其在头颈部鳞状细胞癌发生发展中的作用。
本研究对93例头颈部鳞状细胞癌或发育异常患者的样本进行了研究，这些患者于2015年至2017年在伊朗萨里的布阿里·西纳医院接受了活检或手术。从病理档案中获取相关石蜡包埋组织块，采用免疫组织化学法评估半乳糖凝集素-8。评估半乳糖凝集素-8的表达与年龄、性别、疾病部位和分期之间的关联。为比较各组之间的表达率，使用了麦克内马尔检验、卡方检验和费舍尔精确检验。P值<0.05被认为具有统计学意义。
在97.6%的正常组织病例中观察到强烈的细胞质和细胞核半乳糖凝集素-8染色，而77%的发育异常病变和69%的癌症显示免疫反应阴性。与正常组织相比，发育异常和恶性组织中的表达强度显著降低（P=0.0001）。半乳糖凝集素-8的表达与分期（P=0.303）、淋巴结受累（P=0.326）、肿瘤分级（P=0.769）、远处转移（P=0.748）和年龄（P=0.574）均无相关性。
我们观察到头颈部发育异常和恶性鳞状上皮中半乳糖凝集素-8的表达与它们的正常对应物相比显著降低。它可能有助于头颈部鳞状细胞的恶性转化。
