用于检测南非女性性传播感染的TaqMan探针的性能
Performance of TaqMan probes for the detection of sexually transmitted infections in South African women.
作者信息
Mitchev Nireshni, Singh Ravesh, Garrett Nigel, Ramsuran Veron, Niehaus Abraham J, Mlisana Koleka P
机构信息
Department of Medical Microbiology, School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, South Africa.
Department of Medical Microbiology, National Health Laboratory Service, Durban, South Africa.
出版信息
Afr J Lab Med. 2021 Mar 31;10(1):1124. doi: 10.4102/ajlm.v10i1.1124. eCollection 2021.
Abstract
and are the four main aetiologies of sexually transmitted infections responsible for vaginal discharge syndrome (VDS). Commercially available multiplex polymerase chain reaction (PCR) assays are expensive and generally not customisable. We evaluated a highly customisable singleplex PCR approach by testing it in parallel with the Anyplex™ II STI-7 detection assay in a cohort of South African women that presented with VDS between May 2016 and January 2017. Our multiple singleplex PCR strategy proved to be a simple, accurate, rapid, affordable and scalable option for diagnosing VDS.
摘要
和是导致阴道分泌物综合征（VDS）的性传播感染的四种主要病因。市售的多重聚合酶链反应（PCR）检测方法昂贵且通常无法定制。我们通过在2016年5月至2017年1月期间出现VDS的南非女性队列中，将其与Anyplex™ II STI-7检测方法并行测试，评估了一种高度可定制的单重PCR方法。我们的多重单重PCR策略被证明是诊断VDS的一种简单、准确、快速、经济且可扩展的选择。
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