Institute of Physical Chemistry of the Polish Academy of Sciences, Kasprzaka 44/52, Warsaw, 01-224, Poland.
Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377, München, Germany.
Adv Mater. 2021 Jun;33(24):e2101099. doi: 10.1002/adma.202101099. Epub 2021 May 3.
Graphene is considered a game-changing material, especially for its mechanical and electrical properties. This work exploits that graphene is almost transparent but quenches fluorescence in a range up to ≈40 nm. Graphene as a broadband and unbleachable energy-transfer acceptor without labeling, is used to precisely determine the height of molecules with respect to graphene, to visualize the dynamics of DNA nanostructures, and to determine the orientation of Förster-type resonance energy transfer (FRET) pairs. Using DNA origami nanopositioners, biosensing, single-molecule tracking, and DNA PAINT super-resolution with <3 nm z-resolution are demonstrated. The range of examples shows the potential of graphene-on-glass coverslips as a versatile platform for single-molecule biophysics, biosensing, and super-resolution microscopy.
石墨烯被认为是一种改变游戏规则的材料，特别是因为它具有机械和电气性能。这项工作利用了石墨烯几乎透明但能猝灭荧光的特性，范围可达 ≈40nm。石墨烯作为一种无标记的宽带和不可漂白的能量转移受体，可用于精确确定分子相对于石墨烯的高度，可视化 DNA 纳米结构的动力学，并确定Förster 型共振能量转移（FRET）对的方向。使用 DNA 折纸纳米定位器、生物传感、单分子跟踪和 DNA PAINT 超分辨率（<3nm z 分辨率）进行了演示。这些例子表明，玻璃盖玻片上的石墨烯具有作为单分子生物物理学、生物传感和超分辨率显微镜的多功能平台的潜力。
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