College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, China.
Key Laboratory of Biodiversity Conservation and Sustainable Utilization in Mongolian Plateau for College and University of Inner Mongolia Autonomous Region, Hohhot 010022, China.
Int J Mol Sci. 2024 Sep 25;25(19):10310. doi: 10.3390/ijms251910310.
The gene in the endophytic fungus OW 7.8 isolated from was identified, and the gene knockout mutant Δ was first constructed in this study. Compared with OW 7.8, the Δ mutant exhibited altered colony and mycelia morphology, slower growth rate, and no swainsonine (SW) in mycelia. SW was detected in the gene function complementation strain Δ/, indicating that the function of the gene promoted SW biosynthesis. Six differentially expressed genes (DEGs) closely associated with SW synthesis were identified by transcriptomic analysis of OW 7.8 and Δ, with , , , , and down-regulating, and up-regulating. The expression levels of the six genes were consistent with the transcriptomic analysis results. Five differential metabolites (DEMs) closely associated with SW synthesis were identified by metabolomic analysis, with -glutamate, α-ketoglutaric acid, and -proline up-regulating, and phosphatidic acid (PA) and 2-aminoadipic acid down-regulating. The SW biosynthetic pathways in OW 7.8 were predicted and refined. The results lay the foundation for in-depth elucidation of molecular mechanisms and the SW synthesis pathway in fungi. They are also of importance for the prevention of locoism in livestock, the control and utilization of locoweeds, and the protection and sustainable development of grassland ecosystems.
从内生真菌 OW7.8 中鉴定出一个基因，并首次在本研究中构建了该基因的敲除突变体Δ。与 OW7.8 相比，Δ突变体的菌落和菌丝形态发生了改变，生长速度较慢，且菌丝中没有苦马豆素（SW）。在基因功能互补菌株Δ/中检测到 SW，表明该基因的功能促进了 SW 的生物合成。通过转录组分析 OW7.8 和Δ，鉴定出 6 个与 SW 合成密切相关的差异表达基因（DEGs），其中 、 、 、 、 下调，而上调。这 6 个基因的表达水平与转录组分析结果一致。通过代谢组分析，鉴定出 5 个与 SW 合成密切相关的差异代谢物（DEMs），其中 -谷氨酸、α-酮戊二酸和 -脯氨酸上调，而磷脂酸（PA）和 2-氨基己二酸下调。预测并完善了 OW7.8 中 SW 的生物合成途径。该研究结果为深入阐明真菌中的分子机制和 SW 合成途径奠定了基础，对于预防家畜疯草病、控制和利用疯草、保护和可持续发展草原生态系统具有重要意义。
