意大利南部流浪猫中[具体内容缺失]的血清学和分子证据。
Serological and Molecular Evidence of in Stray Cats from Southern Italy.
作者信息
Grippi Francesca, Galluzzo Paola, Guercio Annalisa, Blanda Valeria, Santangelo Francesco, Sciortino Sonia, Vicari Domenico, Arcuri Francesca, Di Bella Santina, Torina Alessandra
机构信息
Istituto Zooprofilattico Sperimentale della Sicilia "Adelmo Mirri", via G. Marinuzzi 3, 90129 Palermo, Italy.
Dipartimento Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Viale delle Scienze, University of Palermo, 90133 Palermo, Italy.
出版信息
Microorganisms. 2021 Apr 30;9(5):979. doi: 10.3390/microorganisms9050979.
is a slow growing and facultative intracellular pathogen mainly transmitted by arthropod vectors adapted to domestic and wild mammalian reservoir hosts. Since cats are the major source of the infection, this study aimed to evaluate the seroprevalence and the DNA presence in randomly sampled stray cats. Blood samples of 429 cats were collected from shelter of Palermo (Southern Italy) and sera and whole blood were analyzed for the presence of antibodies against by indirect immunofluorescence assay (IFA) and by real-time polymerase chain reaction (PCR), respectively. Two hundred and three sera (47.3%) were positive to IFA and 148 blood samples (34.5%) to real-time PCR. Based on serological results, the evaluation of the potential risk factors (sex, age, coat color) was carried out. The multivariate analysis indicated that cats more than 12 months old were more likely to be seropositive to than cats <12 months. These data will add useful information to the understanding of the spread of in stray cats in Southern Italy.
是一种生长缓慢的兼性细胞内病原体，主要通过适应家养和野生哺乳动物宿主的节肢动物媒介传播。由于猫是主要的感染源，本研究旨在评估随机抽样的流浪猫中的血清阳性率和DNA存在情况。从意大利南部巴勒莫的收容所采集了429只猫的血样，分别通过间接免疫荧光试验（IFA）和实时聚合酶链反应（PCR）分析血清和全血中抗……抗体的存在情况。203份血清（47.3%）IFA检测呈阳性，148份血样（34.5%）实时PCR检测呈阳性。基于血清学结果，对潜在风险因素（性别、年龄、毛色）进行了评估。多变量分析表明，12个月以上的猫比12个月以下的猫血清阳性的可能性更大。这些数据将为了解意大利南部流浪猫中……的传播增添有用信息。
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