Catania Angela Maria, Tomassone Laura, Tarducci Alberto, Grego Elena
Department of Veterinary Science, University of Turin, 10095 Grugliasco, TO, Italy.
Animals (Basel). 2025 Aug 16;15(16):2402. doi: 10.3390/ani15162402.
spp. is a Gram-negative bacterium transmitted by arthropod vectors, implicated in a range of zoonotic infections affecting both humans and animals. Among zoonotic species, is primarily associated with domestic cats and with dogs. In Italy, infections have been reported in both southern and northern regions. This study investigates the presence of spp. in fleas and ticks collected from companion animals in Piedmont region, northwestern Italy. A total of 176 flea and 85 tick specimens were obtained from 92 animals (dogs and cats) between May 2018 and February 2020. Arthropods were morphologically identified using identification keys, and screened for DNA by PCR targeting the rRNA gene. Positive samples were further analyzed by amplifying the gene. Selected -positive and all -positive samples were sequenced and subjected to phylogenetic analysis. Fleas were mostly identified as , recovered from 44 cats and 12 dogs; a single specimen was found in one cat. Ticks were (from 14 cats and 10 dogs), s.l. (3 cats, 7 dogs), and (one dog). prevalence was 38.4%, with 34.2% positivity in fleas and 45.6% in ticks. All sequences corresponded to . These findings confirm the active circulation of in ectoparasites of pets and raise questions about the potential role of ticks in its transmission.
[细菌名称]是一种革兰氏阴性菌，通过节肢动物媒介传播，与一系列影响人类和动物的人畜共患病感染有关。在人畜共患病物种中，[细菌名称]主要与家猫有关，[另一细菌名称]与狗有关。在意大利，南部和北部地区均报告过[细菌名称]感染病例。本研究调查了意大利西北部皮埃蒙特地区从伴侣动物身上采集的跳蚤和蜱中[细菌名称]的存在情况。2018年5月至2020年2月期间，从92只动物（狗和猫）身上共获得了176个跳蚤和85个蜱标本。使用鉴定密钥对节肢动物进行形态学鉴定，并通过针对[细菌名称]rRNA基因的PCR筛选[细菌名称]DNA。对阳性样本进一步扩增[另一基因名称]基因进行分析。对选定的[细菌名称]阳性和所有[另一细菌名称]阳性样本进行测序并进行系统发育分析。跳蚤大多被鉴定为[某种跳蚤名称]，从44只猫和12只狗身上采集到；在一只猫身上发现了一个[另一跳蚤名称]标本。蜱为[某种蜱名称]（来自14只猫和10只狗）、[蜱属名称]狭义种（3只猫，7只狗）和[另一蜱名称]（一只狗）。[细菌名称]患病率为38.4%，跳蚤中的阳性率为34.2%，蜱中的阳性率为45.6%。所有序列均对应于[细菌名称]。这些发现证实了[细菌名称]在宠物外寄生虫中的活跃传播，并引发了关于蜱在其传播中的潜在作用的问题。
