Muñoz-Prieto Alberto, Escribano Damián, Contreras-Aguilar María Dolores, Horvatić Anita, Guillemin Nicolas, Jacobsen Stine, Cerón José Joaquín, Mrljak Vladimir
Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia.
Interdisciplinary Laboratory of Clinical Analysis, Interlab-UMU, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, 30100 Murcia, Spain.
Animals (Basel). 2021 Apr 30;11(5):1304. doi: 10.3390/ani11051304.
The aim of this study was to investigate the changes in the salivary proteome in horses with acute abdominal disease (AAD) using a tandem mass tags (TMT)-based proteomic approach. The saliva samples from eight horses with AAD were compared with six healthy horses in the proteomic study. Additionally, saliva samples from eight horses with AAD and eight controls were used to validate lactoferrin (LF) in saliva. The TMT analysis quantified 118 proteins. Of these, 17 differed significantly between horses with AAD and the healthy controls, 11 being downregulated and 6 upregulated. Our results showed the downregulation of gamma-enteric smooth muscle actin (ACTA2), latherin isoform X1, and LF. These proteins could be closely related to an impaired primary immune defense and antimicrobial capacity in the mucosa. In addition, there was an upregulation of mucin 19 (MUC19) and the serine protease inhibitor Kazal-type 5 (SPINK5) associated with a protective effect during inflammation. The proteins identified in our study could have the potential to be novel biomarkers for diagnosis or monitoring the physiopathology of the disease, especially LF, which decreased in the saliva of horses with AAD and was successfully measured using a commercially available immunoassay.
本研究旨在采用基于串联质谱标签（TMT）的蛋白质组学方法，调查患有急性腹部疾病（AAD）的马匹唾液蛋白质组的变化。在蛋白质组学研究中，将8匹患有AAD的马匹的唾液样本与6匹健康马匹的样本进行了比较。此外，还使用了8匹患有AAD的马匹和8匹对照的唾液样本，来验证唾液中的乳铁蛋白（LF）。TMT分析定量了118种蛋白质。其中，17种在患有AAD的马匹和健康对照之间存在显著差异，11种下调，6种上调。我们的结果显示，γ-肠平滑肌肌动蛋白（ACTA2）、泡沫蛋白异构体X1和LF下调。这些蛋白质可能与黏膜中初级免疫防御和抗菌能力受损密切相关。此外，黏蛋白19（MUC19）和丝氨酸蛋白酶抑制剂Kazal型5（SPINK5）上调，与炎症期间的保护作用相关。我们研究中鉴定出的蛋白质可能有潜力成为诊断或监测该疾病生理病理学的新型生物标志物，尤其是LF，它在患有AAD的马匹唾液中减少，并且使用市售免疫测定法成功进行了测量。
