Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Murcia, Spain.
MED - Mediterranean Institute for Agriculture, Environment and Development & CHANGE - Global Change and Sustainability Institute, University of Évora, Évora, Portugal.
Res Vet Sci. 2024 Feb;167:105112. doi: 10.1016/j.rvsc.2023.105112. Epub 2023 Dec 15.
Equine gastric ulcer syndrome (EGUS) is currently one of the more frequent diseases in horses. We aimed to identify changes in the salivary proteome in horses with EGUS at diagnosis and after successful treatment by using gel proteomics. Saliva samples were collected from nine horses with EGUS before and after treatment and nine matched healthy controls. SDS-PAGE (1DE) and two-dimensional gel electrophoresis (2DE) were performed, and significantly different protein bands and spots were identified by mass spectrometry. Horses with EGUS had increases in proteins such as adenosine deaminase (ADA), triosephosphate isomerase, keratins and immuno-globulin heavy constant mu and decreases in carbonic anhydrase (CA), albumin and prolactin-induced protein. These changes would indicate various physiopathological mechanisms involved in this disease, such as the activation of the immune system, decreased stomach defence mechanisms and inflammation. The treated horses presented lower expression levels of thioredoxin (TRX) after a successful treatment, in proteomics analysis and also measured with a commercially available ELISA kit. Overall, horses with EGUS have protein changes in their saliva when measured with gel proteomics compared with healthy horses, and they also showed changes after successful treatment. These proteins could be potential biomarkers for detection and monitoring treatment response in EGUS.
马属动物溃疡综合征（EGUS）是目前马群中较为常见的疾病之一。本研究旨在应用凝胶蛋白质组学技术，鉴定 EGUS 马在诊断时和成功治疗后的唾液蛋白质组变化。采集 9 例 EGUS 马治疗前后和 9 例匹配健康对照的唾液样本。进行 SDS-PAGE（1DE）和二维凝胶电泳（2DE），并通过质谱鉴定出具有显著差异的蛋白质条带和斑点。EGUS 马的 ADA、磷酸丙糖异构酶、角蛋白和免疫球蛋白重链 μ 等蛋白增加，碳酸酐酶（CA）、白蛋白和催乳素诱导蛋白减少。这些变化表明该疾病涉及各种生理病理机制，如免疫系统激活、胃防御机制减弱和炎症。在成功治疗后，经蛋白质组学分析和市售 ELISA 试剂盒检测，治疗马的硫氧还蛋白（TRX）表达水平降低。与健康马相比，EGUS 马的唾液用凝胶蛋白质组学检测时存在蛋白质变化，治疗后也存在变化。这些蛋白可能是 EGUS 检测和治疗反应监测的潜在生物标志物。
