Centre for Tissue Engineering and Regenerative Medicine, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Kuala Lumpur 56000, Malaysia.
Int J Environ Res Public Health. 2021 Apr 29;18(9):4764. doi: 10.3390/ijerph18094764.
Tissue-engineered substitutes have shown great promise as a potential replacement for current tissue grafts to treat tendon/ligament injury. Herein, we have fabricated aligned polycaprolactone (PCL) and gelatin (GT) nanofibers and further evaluated their physicochemical properties and biocompatibility. PCL and GT were mixed at a ratio of 100:0, 70:30, 50:50, 30:70, 0:100, and electrospun to generate aligned nanofibers. The PCL/GT nanofibers were assessed to determine the diameter, alignment, water contact angle, degradation, and surface chemical analysis. The effects on cells were evaluated through Wharton's jelly-derived mesenchymal stem cell (WJ-MSC) viability, alignment and tenogenic differentiation. The PCL/GT nanofibers were aligned and had a mean fiber diameter within 200-800 nm. Increasing the GT concentration reduced the water contact angle of the nanofibers. GT nanofibers alone degraded fastest, observed only within 2 days. Chemical composition analysis confirmed the presence of PCL and GT in the nanofibers. The WJ-MSCs were aligned and remained viable after 7 days with the PCL/GT nanofibers. Additionally, the PCL/GT nanofibers supported tenogenic differentiation of WJ-MSCs. The fabricated PCL/GT nanofibers have a diameter that closely resembles the native tissue's collagen fibrils and have good biocompatibility. Thus, our study demonstrated the suitability of PCL/GT nanofibers for tendon/ligament tissue engineering applications.
组织工程替代品已显示出巨大的潜力，可以替代当前的组织移植物来治疗肌腱/韧带损伤。在此，我们制备了取向的聚己内酯（PCL）和明胶（GT）纳米纤维，并进一步评估了它们的物理化学性质和生物相容性。将 PCL 和 GT 以 100:0、70:30、50:50、30:70、0:100 的比例混合，并进行静电纺丝以生成取向的纳米纤维。评估了 PCL/GT 纳米纤维的直径、取向、水接触角、降解和表面化学分析。通过 Wharton 胶状基质衍生的间充质干细胞（WJ-MSC）活力、取向和腱形成分化来评估对细胞的影响。PCL/GT 纳米纤维是取向的，平均纤维直径在 200-800nm 之间。增加 GT 浓度会降低纳米纤维的水接触角。单独的 GT 纳米纤维降解最快，仅在 2 天内观察到。化学成分分析证实了纳米纤维中存在 PCL 和 GT。WJ-MSCs 在 PCL/GT 纳米纤维上培养 7 天后仍保持活力且排列整齐。此外，PCL/GT 纳米纤维支持 WJ-MSCs 的腱形成分化。所制备的 PCL/GT 纳米纤维具有与天然组织胶原纤维相似的直径，且具有良好的生物相容性。因此，我们的研究表明 PCL/GT 纳米纤维适用于肌腱/韧带组织工程应用。
