Department of Medical Pharmacology, Faculty of Medicine, İstanbul Medeniyet University, İstanbul, Turkey.
Department of Biochemistry, Faculty of Medicine, İstanbul Medeniyet University, İstanbul, Turkey.
Mol Biol Rep. 2021 Apr;48(4):3205-3212. doi: 10.1007/s11033-021-06377-y. Epub 2021 May 4.
It is known that oxidative stress may cause neuronal injury and several experimental models showed that AsO exposure causes oxidative stress. Lycopene, a carotenoid, has been shown to have protective effect in neurological disease models due to antioxidant activity, but its effect on AsO-induced neurotoxicity is not identified yet. The aim of this study is to investigate the effects of lycopene on AsO-induced neuronal damage and the related mechanisms. Cell viability was determined by the MTT assay. Lycopene was administrated with different concentrations (2, 4, 6 and 8 µM) one hour before 2 µM AsO exposure in SH-SY5Y human neuroblastoma cells. The anti-oxidant effect of lycopene was determined by measuring superoxide dismutase (SOD), catalase (CAT) hydrogen peroxide (HO), malondialdehyde (MDA), total antioxidant status (TAS) and total oxidant status (TOS). MTT results and LDH cytotoxicity analyses showed that pretreatment with 8 µM lycopene significantly improved the toxicity due to AsO exposure in SH‑SY5Y neuroblastoma cells. Pretreatment with lycopene significantly increased the activities of anti‑oxidative enzymes as well as total antioxidant status and decreased total oxidative status in AsO exposed cells. The results of this study indicate that lycopene may be a potent neuroprotective against oxidative stress and could be used to prevent neuronal injury or death in several neurological diseases.
已知氧化应激可能导致神经元损伤，并且几个实验模型表明砷暴露会导致氧化应激。番茄红素作为一种类胡萝卜素，由于其抗氧化活性，已被证明在神经疾病模型中具有保护作用，但它对砷诱导的神经毒性的影响尚未确定。本研究旨在探讨番茄红素对砷诱导的神经元损伤的影响及其相关机制。通过 MTT 测定法测定细胞活力。在 SH-SY5Y 人神经母细胞瘤细胞中暴露于 2 µM 砷之前 1 小时，用不同浓度（2、4、6 和 8 µM）的番茄红素进行处理。通过测量超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化氢（HO）、丙二醛（MDA）、总抗氧化状态（TAS）和总氧化状态（TOS）来确定番茄红素的抗氧化作用。MTT 结果和 LDH 细胞毒性分析表明，8 µM 番茄红素预处理可显著改善 SH-SY5Y 神经母细胞瘤细胞因砷暴露而产生的毒性。番茄红素预处理可显著提高抗氧化酶的活性以及总抗氧化状态，并降低砷暴露细胞中的总氧化状态。本研究结果表明，番茄红素可能是一种有效的抗氧化应激神经保护剂，可用于预防几种神经疾病中的神经元损伤或死亡。
