Aumüller G, Schulze C, Viebahn C
Department of Anatomy and Cell Biology, Philipps-Universität, Marburg, Federal Republic of Germany.
Microsc Res Tech. 1992 Jan 1;20(1):50-72. doi: 10.1002/jemt.1070200107.
Using immunohistochemical techniques both at light and electron microscopic levels, the arrangement and distribution of intermediate filaments in Sertoli cells of normal testis (in rat and human), during pre- and postnatal development (in rabbit, rat, and mouse) and under experimental and pathological conditions (human, rat), have been studied and related to the pertinent literature. Intermediate filaments are centered around the nucleus, where they apparently terminate in the nuclear envelope providing a perinuclear stable core area. From this area they radiate to the plasma membranes; apically often a close association with microtubules is seen. Basally, direct contacts of the filaments with focal adhesions occur, while the relationship to the different junctions of Sertoli cells is only incompletely elucidated. In the rat (not in human) a group of filaments is closely associated with the ectoplasmic specializations surrounding the head of elongating spermatids. Both in rat and human, changes in cell shape during the spermatogenic cycle are associated with a redistribution of intermediate filaments. As inferred from in vitro studies reported in the literature, these changes are at least partly hormone-dependent (vimentin phosphorylation subsequent to FSH stimulation) and influenced by local factors (basal lamina, germ cells). Intermediate filaments, therefore, are suggested to be involved in the hormone-dependent mechanical integration of exogenous and endogenous cell shaping forces. They permit a cycle-dependent compartmentation of the Sertoli cell into a perinuclear stable zone and a peripheral trafficking zone with fluctuating shape. The latter is important with respect to the germ cell-supporting surface of the cell which seems to limit the spermatogenetic potential of the male gonad.
运用光镜和电镜水平的免疫组织化学技术，对正常睾丸（大鼠和人类）、出生前后发育阶段（兔、大鼠和小鼠）以及实验和病理条件下（人类、大鼠）支持细胞中中间丝的排列和分布进行了研究，并与相关文献进行了关联。中间丝围绕细胞核分布，在核膜处明显终止，形成一个核周稳定核心区域。从这个区域它们向质膜放射状延伸；在顶端常可见与微管紧密相连。在基部，丝与粘着斑直接接触，而与支持细胞不同连接的关系仅得到部分阐明。在大鼠（而非人类）中，一组丝与伸长精子头部周围的外质特化结构紧密相关。在大鼠和人类中，生精周期中细胞形状的变化都与中间丝的重新分布有关。根据文献报道的体外研究推断，这些变化至少部分依赖激素（促卵泡激素刺激后波形蛋白磷酸化），并受局部因素（基膜、生殖细胞）影响。因此，提示中间丝参与了激素依赖的外源性和内源性细胞塑形力的机械整合。它们使支持细胞能够进行周期性分隔，分为核周稳定区和形状波动的外周运输区。后者对于细胞的生殖细胞支持表面很重要，而这似乎限制了雄性性腺的生精潜能。
