Faculdade de Ciências Agrárias e Veterinárias - FCAV, Universidade Estadual Paulista - UNESP, Jaboticabal, SP, Brasil.
Centro Universitário Central Paulista - UNICEP, São Carlos, SP, Brasil.
Rev Bras Parasitol Vet. 2021 May 3;30(2):e025120. doi: 10.1590/S1984-296120201093. eCollection 2021.
This study evaluated the resistance status of Haemonchus contortus from sheep flocks in the state of São Paulo, Brazil, through comparison between the fecal egg count reduction test (FECRT) and the larval development test (LDT). For the FECRT, 35 sheep were selected in each of five flocks and divided into groups treated with: benzimidazole, levamisole, ivermectin, monepantel and control. Feces were collected for EPG and fecal cultures. The LDT was performed using thiabendazole (TBZ), levamisole (LEV), ivermectin aglycone (IVM-A) and Zolvix (ZLV). Resistance to all drugs was detected using FECRT in 100% of the flocks, except in relation to ZLV (40% resistant and 20% suspected of resistance). LDT indicated resistance to TBZ and IVM-A in all flocks, to LEV in 80% of flocks and to ZLV in 10%. Total agreement was obtained between the two tests for TBZ and IVM (k = 1.0), while for LEV (k = 0.8) and ZLV (k = 0.9), substantial and almost perfect agreement were obtained, respectively. The concordance between the tests was significant, thus showing that it is possible to use the outcome of the LDT to predict the FECRT, and hence validating the former as a fast diagnostic test for use by sheep farmers in Brazil.
本研究通过比较粪便卵囊减少试验（FECRT）和幼虫发育试验（LDT），评估了巴西圣保罗州绵羊群中捻转血矛线虫的耐药性。在 FECRT 中，从每个羊群中选择 35 只绵羊，并将其分为以下治疗组：苯并咪唑类、左旋咪唑、伊维菌素、莫能菌素和对照组。采集粪便进行 EPG 和粪便培养。使用噻苯达唑（TBZ）、左旋咪唑（LEV）、伊维菌素糖苷（IVM-A）和 Zolvix（ZLV）进行 LDT。除了 ZLV（40%耐药和 20%疑似耐药）外，FECRT 检测到所有羊群对所有药物的耐药性。LDT 表明所有羊群对 TBZ 和 IVM-A 耐药，80%的羊群对 LEV 耐药，10%的羊群对 ZLV 耐药。TBZ 和 IVM 的两种检测方法完全一致（k = 1.0），而 LEV（k = 0.8）和 ZLV（k = 0.9）的检测方法则具有高度一致性和几乎完全一致性。两种检测方法的一致性显著，表明可以使用 LDT 的结果来预测 FECRT，从而验证了前者作为巴西绵羊养殖户快速诊断试验的有效性。
